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          • Primary Antibodies ›
          • GFP Antibodies

          Proteintech

          GFP tag Monoclonal Antibody (1E10H7), CoraLite® Plus 488

          View all (127) GFP antibodies

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          Cite GFP tag Monoclonal Antibody (1E10H7), CoraLite® Plus 488

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          • Antibody Testing Data (1)
          GFP tag Antibody in Western Blot (WB)
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          GFP tag Antibody in Western Blot (WB)
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          FIGURE: 1 / 1

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          GFP tag Antibody (CL48866002100UL) in WB

          Various lysates (Recombinant protein) were subjected to SDS PAGE followed by western blot with (Product # CL48866002100UL) (GFP tag antibody) at dilution of 1:5,000 incubated at room temperature for 1.5 hours. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          GFP tag Antibody in Western Blot (WB)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Product Details

          CL48866002100UL

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:2,000-1:10,000
          -

          Immunocytochemistry (ICC/IF)

          1:50-1:500
          -
          Product Specifications

          Species Reactivity

          Tag

          Host/Isotype

          Mouse / IgG2a

          Class

          Monoclonal

          Type

          Antibody

          Clone

          1E10H7

          Conjugate

          CoraLite® Plus 488 CoraLite® Plus 488 CoraLite® Plus 488
          • Unconjugated
          • CoraLite 594
          • CoraLite Plus 647
          • HRP
          • Request custom conjugation

          Excitation/Emission Max

          493/522 nm

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Protein A

          Storage buffer

          PBS, pH 7.3, with 50% glycerol, 0.5% BSA

          Contains

          0.05% ProClin 300

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles, store in dark

          Shipping conditions

          Wet ice

          Target Information

          The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: GFP tag; GFP2; green fluorescence; green fluorescent; Turbo eGFP

          View more View less

          It has to be done as per old AB suggested Products section.

          Disclaimer

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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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