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FIGURE: 1 / 1
Human BMP-4 Protein (PHC9531) in Bioactivity
Recombinant human BMP-4 (Product # PHC9531) stimulates dose-dependent induction of alkaline phosphatase production in the ATDC-5 mouse chondrogenic cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. ATDC-5 cells were treated with increasing concentrations of recombinant human BMP-4 for 72 hrs hours before lysis and addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values r... View More
Product Details
PHC9531
Applications
Tested Dilution
Publications
Product Specifications
Species
Human,
Mouse
Published species
Human,
Mouse
Expression System
HEK293 cells
Molecular weight
34 kDa
Class
Recombinant
Type
Protein
Purity
>95% by SDS-PAGE
Endotoxin concentration
<1 EU/µg
Activity
ED50 = 2 - 10 ng/mL; determined by the dose-dependent induction of alkaline phosphatase production in ATDC-5 cells.
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
HCl
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Dry ice
Product Specific Information
Carrier-Free
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute lyophilized recombinant human BMP-4 in 4 mM HCl containing 0.1% human serum albumin. Further dilutions should be made in low endotoxin medium or a buffered solution containing a carrier protein such as heat inactivated FCS or tissue culture grade BSA.
Storage: Store this lyophilized preparation at ≤-20°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at ≤-20°C. Avoid repeated freeze/thaw cycles.
Target Information
The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. This particular family member plays an important role in the onset of endochondral bone formation in humans, and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing in the 5' untranslated region of this gene has been described and three variants are described, all encoding an identical protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: BMP; BMP-2B; BMP-4; Bone morphogenetic protein; Bone morphogenetic protein 2B; Bone morphogenetic protein 4; bone morphogenetic protein 4 preproprotein
Gene Aliases: Bmp-4; BMP2B; Bmp2b-1; BMP2B1; BMP4; Dvr-4; DVR4; MCOPS6; OFC11; ZYME
UniProt ID: (Human) P12644, (Mouse) P21275
Entrez Gene ID: (Human) 652, (Mouse) 12159
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