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Abnova
IMPACT Polyclonal Antibody, MaxPab™
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FIGURE: 1 / 2
IMPACT Antibody (H00055364-B01P) in WB
Western Blot analysis of IMPACT expression in transfected 293T cell line (H00055364-T01) by IMPACT MaxPab polyclonal antibody.
Lane 1: IMPACT transfected lysate(35.2 KDa).
Lane 2: Non-transfected lysate.
Lane 1: IMPACT transfected lysate(35.2 KDa).
Lane 2: Non-transfected lysate.
Product Details
H00055364-B01P
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Mouse
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
IMPACT (AAH34016, 1 a.a. approximately 320 a.a) full-length human protein.
Conjugate
Form
Liquid
Concentration
See Label
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Sequence of this protein is as follows: MAEGDAGSDQ RQNEEIEAMA AIYGEEWCVI DDCAKIFCIR ISDDIDDPKW TLCLQVMLPN EYPGTAPPIY QLNAPWLKGQ ERADLSNSLE EIYIQNIGES ILYLWVEKIR DVLIQKSQMT EPGPDVKKKT EEEDVECEDD LILACQPESS VKALDFDISE TRTEVEVEEL PPIDHGIPIT DRRSTFQAHL APVVCPKQVK MVLSKLYENK KIASATHNIY AYRIYCEDKQ TFLQDCEDDG ETAAGGRLLH LMEILNVKNV MVVVSRWYGG ILLGPDRFKH INNCARNILV EKNYTNSPEE SSKALGKNKK VRKDKKRNEH
Target Information
Translational regulator that ensures constant high levels of translation upon a variety of stress conditions, such as amino acid starvation, UV-C irradiation, proteasome inhibitor treatment and glucose deprivation. Plays a role as a negative regulator of the EIF2AK4/GCN2 kinase activity; impairs GCN1-mediated EIF2AK4/GCN2 activation, and hence EIF2AK4/GCN2-mediated eIF-2-alpha phosphorylation and subsequent down-regulation of protein synthesis. May be required to regulate translation in specific neuronal cells under amino acid starvation conditions by preventing GCN2 activation and therefore ATF4 synthesis. Through its inhibitory action on EIF2AK4/GCN2, plays a role in differentiation of neuronal cells by stimulating neurite outgrowth.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Impact homolog; Imprinted and ancient gene protein homolog; Protein IMPACT; RWD domain containing 5
Gene Aliases: IMPACT; RWDD5
UniProt ID: (Human) Q9P2X3, (Rat) Q5GFD9
Entrez Gene ID: (Human) 55364, (Rat) 497198
negative regulation of protein phosphorylation
regulation of translational initiation
negative regulation of transcription from RNA polymerase II promoter
biological_process
negative regulation of protein complex assembly
negative regulation of protein autophosphorylation
cellular response to amino acid starvation
cellular response to glucose starvation
positive regulation of neuron differentiation
negative regulation of cell death
regulation of eIF2 alpha phosphorylation by amino acid starvation
cellular response to hydrogen peroxide
positive regulation of translational initiation in response to starvation
cellular response to acidic pH
cellular response to UV-C
cellular response to benomyl
negative regulation of transcription from RNA polymerase II promoter in response to stress
neuron projection extension
cellular response to leucine starvation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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