Invitrogen

IMPDH2 Polyclonal Antibody

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IMPDH2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of human IMPDH2 in human placenta with IMPDH2 Polyclonal Antibody (Product # PA5-118010, 1:100 dilution, formalin-fixed paraffin embedded sections).

IMPDH2 Antibody in Western Blot (WB) — Western Blot using IMPDH2 Polyclonal Antibody (Product # PA5-118010) at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 56 kDa. Observed band size: 56 kDa.

IMPDH2 Antibody in Immunoprecipitation (IP) — IMPDH2 Immunoprecipitation using: Lane A: 0.5 mg HeLa Whole Cell Lysate 4 µL with IMPDH2 Polyclonal Antibody (Product # PA5-118010) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: IMPDH2 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1,000dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 56 kDa. Observed band size: 60 kDa.

Product Details

PA5-118010

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunoprecipitation (IP)	1-5 μL/mg of lysate	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	E. coli-derived Human IMPDH2 fragment
Conjugate	Unconjugated
Form	Liquid
Purification	Antigen affinity chromatography, Protein A
Storage buffer	PBS, pH 7
Contains	0.03% Proclin 300
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Target Information

This gene encodes the rate-limiting enzyme in the de novo guanine nucleotide biosynthesis. It is thus involved in maintaining cellular guanine deoxy- and ribonucleotide pools needed for DNA and RNA synthesis. The encoded protein catalyzes the NAD-dependent oxidation of inosine-5'-monophosphate into xanthine-5'-monophosphate, which is then converted into guanosine-5'-monophosphate. This gene is up-regulated in some neoplasms, suggesting it may play a role in malignant transformation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: IMP (inosine 5'-monophosphate) dehydrogenase 2; IMP (inosine monophosphate) dehydrogenase 2; IMP dehydrogenase 2; IMP oxireductase 2; IMPD 2; IMPDH 2; IMPDH II; IMPDH-II; IMPDH2; inosine 5' phosphate dehydrogenase 2; inosine monophosphate dehydrogenase type II; Inosine-5'-monophosphate dehydrogenase 2

Gene Aliases: IMPD2; IMPDH-II; IMPDH2

UniProt ID: (Human) P12268

Entrez Gene ID: (Human) 3615

Molecular Function: dehydrogenase