Proteintech

ING4 Polyclonal Antibody

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Antibody Testing Data (6)

FIGURE: 1 / 6

ING4 Antibody (10617-1-AP) in WB

Various lysates were subjected to SDS PAGE followed by western blot with (Product # 10617-1-AP) ING4 antibody at dilution of 1:8,000 incubated at room temperature for 1.5 hours.

ING4 Antibody in Immunoprecipitation (IP)

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Product Details

10617-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 6 publications 6 publications

Immunohistochemistry (IHC)

Assay-dependent

View 8 publications 8 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ING4 Fusion Protein Ag0960 (1-249 aa encoded by BC007781)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody is a rabbit polyclonal antibody raised against full length human ING4 antigen.

Immunogen sequence: MAAGMYLEH YLDSIENLPF ELQRNFQLMR DLDQRTEDLK AEIDKLATEY MSSARSLSSE EKLALLKQIQ EAYGKCKEFG DDKVQLAMQT YEMVDKHIRR LDTDLARFEA DLKEKQIESS DYDSSSSKGK KKGRTQKEKK AARARSKGKN SDEEAPKTAQ KKLKLVRTSP EYGMPSVTFG SVHPSDVLDM PVDPNEPTYC LCHQVSYGEM IGCDNPDCSI EWFHFACVGL TTKPRGKWFC PRCSQERKKK (1-249 aa encoded by BC007781)

Target Information

The ING4 gene belongs to the ING (Inhibitor of Growth) family of tumor suppressors. All five members of the ING protein family identified to date contain a highly conserved plant homeodomain (PHD) finger motif at the C-terminal end, which is found in transcription factors that modulate chromatin structure. ING proteins have been shown to form transcriptional complexes leading to activation of responsive genes that mediate a wide range of cellular functions, including growth arrest, DNA repair, gene transcription, apoptosis, and senescence. The candidate tumor suppressor ING4 has a role in brain tumor pathogenesis, and is involved in regulating tumor growth and angiogenesis. Expression of ING4 is reduced in gliomas compared to normal brain tissue, and ING4 reduction correlates with progression from lower to higher grades of tumors. ING4 physically interacts with the p65 (RelA) subunit of NF-kB and represses transcription of NF-kB responsive genes. Inactivating mutations in ING4 transcripts have been described in various human cancer cell lines, and deletion of the ING4 locus has been detected in 10-20% of human breast cancer cell lines and tumors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Aberrant CREB1 activation in prostate cancer disrupts normal prostate luminal cell differentiation.

Oncogene

Watson MJ,Berger PL,Banerjee K,Frank SB,Tang L,Ganguly SS,Hostetter G,Winn M,Miranti CK

Sat May 01 00:00:00 EDT 2021

ING4 alleviated lipopolysaccharide-induced inflammation by regulating the NF-κB pathway via a direct interaction with SIRT1.

Immunology and cell biology

Yang Y,Liu Y,He X,Yang F,Han S,Qin A,Wu G,Liu M,Li Z,Wang J,Yang X,Hu D

Sat Feb 01 00:00:00 EST 2020

MicroRNA‑214 upregulates HIF‑1α and VEGF by targeting ING4 in lung cancer cells.

Molecular medicine reports

Li Y,Zhao L,Qi Y,Yang X

Sat Jun 01 00:00:00 EDT 2019

ING4 enhances paclitaxel's effect on colorectal cancer growth in vitro and in vivo.

International journal of clinical and experimental pathology

Cao L,Chen S,Zhang C,Chen C,Lu N,Jiang Y,Cai Y,Yin Y,Xu J

Mon May 16 00:00:00 EDT 2016

Transient induction of ING4 by Myc drives prostate epithelial cell differentiation and its disruption drives prostate tumorigenesis.

Cancer research

Berger PL,Frank SB,Schulz VV,Nollet EA,Edick MJ,Holly B,Chang TT,Hostetter G,Kim S,Miranti CK

Sun Jun 15 00:00:00 EDT 2014

Transient induction of ING4 by Myc drives prostate epithelial cell differentiation and its disruption drives prostate tumorigenesis.

Cancer research

Berger PL,Frank SB,Schulz VV,Nollet EA,Edick MJ,Holly B,Chang TT,Hostetter G,Kim S,Miranti CK

Sun Jun 15 00:00:00 EDT 2014

Expression of hypoxia-inducible factor (HIF)-1a-vascular endothelial growth factor (VEGF)-inhibitory growth factor (ING)-4- axis in sarcoidosis patients.

BMC research notes

Tzouvelekis A,Ntolios P,Karameris A,Koutsopoulos A,Boglou P,Koulelidis A,Archontogeorgis K,Zacharis G,Drakopanagiotakis F,Steiropoulos P,Anevlavis S,Polychronopoulos V,Mikroulis D,Bouros D

Mon Nov 26 00:00:00 EST 2012

Expression of hypoxia-inducible factor (HIF)-1a-vascular endothelial growth factor (VEGF)-inhibitory growth factor (ING)-4- axis in sarcoidosis patients.

BMC research notes

Tzouvelekis A,Ntolios P,Karameris A,Koutsopoulos A,Boglou P,Koulelidis A,Archontogeorgis K,Zacharis G,Drakopanagiotakis F,Steiropoulos P,Anevlavis S,Polychronopoulos V,Mikroulis D,Bouros D

Mon Nov 26 00:00:00 EST 2012

The Hbo1-Brd1/Brpf2 complex is responsible for global acetylation of H3K14 and required for fetal liver erythropoiesis.

Blood

Mishima Y,Miyagi S,Saraya A,Negishi M,Endoh M,Endo TA,Toyoda T,Shinga J,Katsumoto T,Chiba T,Yamaguchi N,Kitabayashi I,Koseki H,Iwama A

Thu Sep 01 00:00:00 EDT 2011

The Hbo1-Brd1/Brpf2 complex is responsible for global acetylation of H3K14 and required for fetal liver erythropoiesis.

Blood

Mishima Y,Miyagi S,Saraya A,Negishi M,Endoh M,Endo TA,Toyoda T,Shinga J,Katsumoto T,Chiba T,Yamaguchi N,Kitabayashi I,Koseki H,Iwama A

Thu Sep 01 00:00:00 EDT 2011

Downregulated expression of inhibitor of growth 4 (ING4) in advanced colorectal cancers: a non-randomized experimental study.

Pathology oncology research : POR

You Q,Wang XS,Fu SB,Jin XM

Thu Sep 01 00:00:00 EDT 2011

Downregulated expression of inhibitor of growth 4 (ING4) in advanced colorectal cancers: a non-randomized experimental study.

Pathology oncology research : POR

You Q,Wang XS,Fu SB,Jin XM

Thu Sep 01 00:00:00 EDT 2011

Loss of inhibitor of growth (ING-4) is implicated in the pathogenesis and progression of human astrocytomas.

Brain pathology (Zurich, Switzerland)

Klironomos G,Bravou V,Papachristou DJ,Gatzounis G,Varakis J,Parassi E,Repanti M,Papadaki H

Mon Mar 01 00:00:00 EST 2010

Loss of inhibitor of growth (ING-4) is implicated in the pathogenesis and progression of human astrocytomas.

Brain pathology (Zurich, Switzerland)

Klironomos G,Bravou V,Papachristou DJ,Gatzounis G,Varakis J,Parassi E,Repanti M,Papadaki H

Mon Mar 01 00:00:00 EST 2010

Down-regulation of the inhibitor of growth family member 4 (ING4) in different forms of pulmonary fibrosis.

Respiratory research

Tzouvelekis A,Aidinis V,Harokopos V,Karameris A,Zacharis G,Mikroulis D,Konstantinou F,Steiropoulos P,Sotiriou I,Froudarakis M,Pneumatikos I,Tringidou R,Bouros D

Fri Feb 27 00:00:00 EST 2009

Down-regulation of the inhibitor of growth family member 4 (ING4) in different forms of pulmonary fibrosis.

Respiratory research

Tzouvelekis A,Aidinis V,Harokopos V,Karameris A,Zacharis G,Mikroulis D,Konstantinou F,Steiropoulos P,Sotiriou I,Froudarakis M,Pneumatikos I,Tringidou R,Bouros D

Fri Feb 27 00:00:00 EST 2009

Down-regulation of the inhibitor of growth family member 4 (ING4) in different forms of pulmonary fibrosis.

Respiratory research

Tzouvelekis A,Aidinis V,Harokopos V,Karameris A,Zacharis G,Mikroulis D,Konstantinou F,Steiropoulos P,Sotiriou I,Froudarakis M,Pneumatikos I,Tringidou R,Bouros D

Fri Feb 27 00:00:00 EST 2009

The ING4 tumor suppressor attenuates NF-kappaB activity at the promoters of target genes.

Molecular and cellular biology