|Tested species reactivity||Human, Mouse|
|Published species reactivity||Mouse, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized peptide derived from the C-terminal region of human JAK2. The sequence is conserved in mouse and rat.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 50% glycerol, 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Janus Activating Kinase 2 (JAK2) is one of a number of related tyrosine kinases involved in cytoplasmic signal transduction. In response to a varitety of cytokine or related factors (e.g., interferon, interleukins), JAKs are activated via phosphorylation at 2 adjacent tyrosine residues. The activation of JAKs can lead to the phosphorylation of STAT (signal transducers and activatiors of transcription) proteins, which dimerize and translocate to the nucleus. Once translocated to the nucleus, the STAT proteins can modify transcription of numerous genes, including interferon-stimulated genes. JAK2 is required for the IFN gamma-receptor complex initiation and JAK1 functions as an amplifier. However, active JAK1 may be required for complex responses. Some studies have suggested that the role of JAK2 might be performed by Tyk2 and JAK3, if they were positioned correctly within the IFN gamma-receptor complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Mouse||Not Cited||Glia maturation factor regulation of STAT expression: a novel mechanism in experimental autoimmune encephalomyelitis.||Zaheer S,Wu Y,Bassett J,Yang B,Zaheer A||Neurochemical research (32:2123)||2007|
|Mouse||Not Cited||Stat3 is required for full neoplastic transformation by the Simian Virus 40 large tumor antigen.||Vultur A,Arulanandam R,Turkson J,Niu G,Jove R,Raptis L||Molecular biology of the cell (16:3832)||2005|
|Mouse||Not Cited||Protein tyrosine phosphatase 1B negatively regulates leptin signaling in a hypothalamic cell line.||Kaszubska W,Falls HD,Schaefer VG,Haasch D,Frost L,Hessler P,Kroeger PE,White DW,Jirousek MR,Trevillyan JM||Molecular and cellular endocrinology (195:109)||2002|
|Human||Not Cited||The serine protease plasmin triggers expression of MCP-1 and CD40 in human primary monocytes via activation of p38 MAPK and janus kinase (JAK)/STAT signaling pathways.||Burysek L,Syrovets T,Simmet T||The Journal of biological chemistry (277:33509)||2002|
||The serine protease plasmin triggers expression of MCP-1 and CD40 in human primary monocytes via activation of p38 MAPK and janus kinase (JAK)/STAT signaling pathways.||Burysek L,Syrovets T,Simmet T||The Journal of biological chemistry (277:33509)||2002|