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KARS Polyclonal Antibody

KARS Antibody in Immunocytochemistry (ICC/IF)

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KARS Antibody (14951-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 14951-1-AP (KARS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

KARS Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

14951-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Zebrafish

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KARS Fusion Protein Ag6778 (246-597 aa encoded by BC004132)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.31 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: IRSKIITYI RSFLDELGFL EIETPMMNII PGGAVAKPFI TYHNELDMNL YMRIAPELYH KMLVVGGIDR VYEIGRQFRN EGIDLTHNPE FTTCEFYMAY ADYHDLMEIT EKMVSGMVKH ITGSYKVTYH PDGPEGQAYD VDFTPPFRRI NMVEELEKAL GMKLPETNLF ETEETRKILD DICVAKAVEC PPPRTTARLL DKLVGEFLEV TCINPTFICD HPQIMSPLAK WHRSKEGLTE RFELFVMKKE ICNAYTELND PMRQRQLFEE QAKAKAAGDD EAMFIDENFC TALEYGLPPT AGWGMGIDRV AMFLTDSNNI KEVLLFPAMK PEDKKENVAT TDTLESTTVG TSV (246-597 aa encoded by BC004132)

Target Information

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Multimodal cotranslational interactions direct assembly of the human multi-tRNA synthetase complex.

Proceedings of the National Academy of Sciences of the United States of America

Khan K,Long B,Gogonea V,Deshpande GM,Vasu K,Fox PL

Tue Sep 06 00:00:00 UTC 2022

Multimodal cotranslational interactions direct assembly of the human multi-tRNA synthetase complex.

Proceedings of the National Academy of Sciences of the United States of America

Khan K,Long B,Gogonea V,Deshpande GM,Vasu K,Fox PL

Tue Sep 06 00:00:00 UTC 2022

Regulation of ex-translational activities is the primary function of the multi-tRNA synthetase complex.

Nucleic acids research

Cui H,Kapur M,Diedrich JK,Yates JR,Ackerman SL,Schimmel P

Mon Apr 19 00:00:00 UTC 2021

Leopard-like retinopathy and severe early-onset portal hypertension expand the phenotype of KARS1-related syndrome: a case report.

BMC medical genomics

Peluso F,Palazzo V,Indolfi G,Mari F,Pasqualetti R,Procopio E,Nesti C,Guerrini R,Santorelli F,Giglio S

Thu Jan 21 00:00:00 UTC 2021

Lysyl-tRNA synthetase produces diadenosine tetraphosphate to curb STING-dependent inflammation.

Science advances

Guerra J,Valadao AL,Vlachakis D,Polak K,Vila IK,Taffoni C,Prabakaran T,Marriott AS,Kaczmarek R,Houel A,Auzemery B,Déjardin S,Boudinot P,Nawrot B,Jones NJ,Paludan SR,Kossida S,Langevin C,Laguette N

Fri May 01 00:00:00 UTC 2020

Deletion of Gtpbp3 in zebrafish revealed the hypertrophic cardiomyopathy manifested by aberrant mitochondrial tRNA metabolism.

Nucleic acids research

Chen D,Zhang Z,Chen C,Yao S,Yang Q,Li F,He X,Ai C,Wang M,Guan MX

Tue Jun 04 00:00:00 UTC 2019

Deletion of Mtu1 (Trmu) in zebrafish revealed the essential role of tRNA modification in mitochondrial biogenesis and hearing function.

Nucleic acids research

Zhang Q,Zhang L,Chen D,He X,Yao S,Zhang Z,Chen Y,Guan MX

Fri Nov 16 00:00:00 UTC 2018

Deletion of Mtu1 (Trmu) in zebrafish revealed the essential role of tRNA modification in mitochondrial biogenesis and hearing function.

Nucleic acids research

Zhang Q,Zhang L,Chen D,He X,Yao S,Zhang Z,Chen Y,Guan MX

Fri Nov 16 00:00:00 UTC 2018

Inhibition of mitochondrial translation in fibroblasts from a patient expressing the KARS p.(Pro228Leu) variant and presenting with sensorineural deafness, developmental delay, and lactic acidosis.

Human mutation

Ruzzenente B,Assouline Z,Barcia G,Rio M,Boddaert N,Munnich A,Rötig A,Metodiev MD

Sat Dec 01 00:00:00 UTC 2018

Inhibition of mitochondrial translation in fibroblasts from a patient expressing the KARS p.(Pro228Leu) variant and presenting with sensorineural deafness, developmental delay, and lactic acidosis.

Human mutation

Ruzzenente B,Assouline Z,Barcia G,Rio M,Boddaert N,Munnich A,Rötig A,Metodiev MD

Sat Dec 01 00:00:00 UTC 2018

Novel RNA-Affinity Proteogenomics Dissects Tumor Heterogeneity for Revealing Personalized Markers in Precision Prognosis of Cancer.

Cell chemical biology

Wang L,Wrobel JA,Xie L,Li D,Zurlo G,Shen H,Yang P,Wang Z,Peng Y,Gunawardena HP,Zhang Q,Chen X

Thu May 17 00:00:00 UTC 2018

Novel RNA-Affinity Proteogenomics Dissects Tumor Heterogeneity for Revealing Personalized Markers in Precision Prognosis of Cancer.

Cell chemical biology

Wang L,Wrobel JA,Xie L,Li D,Zurlo G,Shen H,Yang P,Wang Z,Peng Y,Gunawardena HP,Zhang Q,Chen X

Thu May 17 00:00:00 UTC 2018

Novel mutations in KARS cause hypertrophic cardiomyopathy and combined mitochondrial respiratory chain defect.

Clinical genetics

Verrigni D,Diodato D,Di Nottia M,Torraco A,Bellacchio E,Rizza T,Tozzi G,Verardo M,Piemonte F,Tasca G,D'Amico A,Bertini E,Carrozzo R

Thu Jun 01 00:00:00 UTC 2017

Novel mutations in KARS cause hypertrophic cardiomyopathy and combined mitochondrial respiratory chain defect.

Clinical genetics

Verrigni D,Diodato D,Di Nottia M,Torraco A,Bellacchio E,Rizza T,Tozzi G,Verardo M,Piemonte F,Tasca G,D'Amico A,Bertini E,Carrozzo R

Thu Jun 01 00:00:00 UTC 2017

Ehrlichia chaffeensis Tandem Repeat Effector Targets Differentially Influence Infection.

Frontiers in cellular and infection microbiology

Luo T,Dunphy PS,McBride JW

Thu Jan 11 00:00:00 UTC 2018

Ehrlichia chaffeensis Tandem Repeat Effector Targets Differentially Influence Infection.

Frontiers in cellular and infection microbiology

Luo T,Dunphy PS,McBride JW

Thu Jan 11 00:00:00 UTC 2018

Bioinformatics

Protein Aliases: EC 6.1.1.6; lysine tRNA ligase; Lysine--tRNA ligase; LysRS; Lysyl-tRNA synthetase

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Gene Aliases: CMTRIB; DFNB89; KARS; KARS1; KARS2; KIAA0070; KRS

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UniProt ID: (Human) Q15046

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Entrez Gene ID: (Human) 3735

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Function(s)

tRNA binding lysine-tRNA ligase activity protein binding ATP binding amino acid binding metal ion binding aminoacyl-tRNA synthetase

Process(es)

tRNA aminoacylation for protein translation lysyl-tRNA aminoacylation tRNA processing diadenosine tetraphosphate biosynthetic process viral process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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KARS Polyclonal Antibody

KARS Antibody (14951-1-AP) in ICC/IF

Product Details

14951-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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