Proteintech

KARS Polyclonal Antibody

View all (18) KARS antibodies

Questions & Answers

Questions & Answers

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KARS Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 14951-1-AP (KARS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

KARS Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human placenta using 14951-1-AP (KARS antibody) at dilution of 1:100 (under 40x lens).

KARS Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human placenta using 14951-1-AP (KARS antibody) at dilution of 1:100 (under 10x lens).

KARS Antibody in Western Blot (WB) — Human colon tissue were subjected to SDS PAGE followed by western blot with 14951-1-AP (KARS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

KARS Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with 14951-1-AP (KARS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

KARS Antibody in Western Blot (WB) — Jurkat cells were subjected to SDS PAGE followed by western blot with 14951-1-AP (KARS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Product Details

14951-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:4,000	View 4 publications 4 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:500	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Zebrafish
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	KARS Fusion Protein Ag6778 (246-597 aa encoded by BC004132) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.31 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: IRSKIITYI RSFLDELGFL EIETPMMNII PGGAVAKPFI TYHNELDMNL YMRIAPELYH KMLVVGGIDR VYEIGRQFRN EGIDLTHNPE FTTCEFYMAY ADYHDLMEIT EKMVSGMVKH ITGSYKVTYH PDGPEGQAYD VDFTPPFRRI NMVEELEKAL GMKLPETNLF ETEETRKILD DICVAKAVEC PPPRTTARLL DKLVGEFLEV TCINPTFICD HPQIMSPLAK WHRSKEGLTE RFELFVMKKE ICNAYTELND PMRQRQLFEE QAKAKAAGDD EAMFIDENFC TALEYGLPPT AGWGMGIDRV AMFLTDSNNI KEVLLFPAMK PEDKKENVAT TDTLESTTVG TSV (246-597 aa encoded by BC004132)

Target Information

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Inhibition of mitochondrial translation in fibroblasts from a patient expressing the KARS p.(Pro228Leu) variant and presenting with sensorineural deafness, developmental delay, and lactic acidosis.

Human mutation

Ruzzenente B,Assouline Z,Barcia G,Rio M,Boddaert N,Munnich A,Rötig A,Metodiev MD

Sat Dec 01 00:00:00 EST 2018

Deletion of Mtu1 (Trmu) in zebrafish revealed the essential role of tRNA modification in mitochondrial biogenesis and hearing function.

Nucleic acids research

Zhang Q,Zhang L,Chen D,He X,Yao S,Zhang Z,Chen Y,Guan MX

Fri Nov 16 00:00:00 EST 2018

Novel RNA-Affinity Proteogenomics Dissects Tumor Heterogeneity for Revealing Personalized Markers in Precision Prognosis of Cancer.

Cell chemical biology

Wang L,Wrobel JA,Xie L,Li D,Zurlo G,Shen H,Yang P,Wang Z,Peng Y,Gunawardena HP,Zhang Q,Chen X

Thu May 17 00:00:00 EDT 2018

Ehrlichia chaffeensis Tandem Repeat Effector Targets Differentially Influence Infection.

Frontiers in cellular and infection microbiology

Luo T,Dunphy PS,McBride JW

Thu Jan 11 00:00:00 EST 2018

Novel mutations in KARS cause hypertrophic cardiomyopathy and combined mitochondrial respiratory chain defect.

Clinical genetics

Verrigni D,Diodato D,Di Nottia M,Torraco A,Bellacchio E,Rizza T,Tozzi G,Verardo M,Piemonte F,Tasca G,D'Amico A,Bertini E,Carrozzo R

Thu Jun 01 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: EC 6.1.1.6; lysine tRNA ligase; Lysine--tRNA ligase; LysRS; Lysyl-tRNA synthetase

Gene Aliases: AA589550; AL024334; AL033315; AL033367; CMTRIB; D8Ertd698e; D8Wsu108e; DFNB89; KARS; KARS1; KARS2; KIAA0070; KRS; LysRS; mKIAA0070

UniProt ID: (Human) Q15046, (Rat) Q5XIM7, (Mouse) Q99MN1

Entrez Gene ID: (Human) 3735, (Rat) 292028, (Mouse) 85305

Molecular Function: aminoacyl-tRNA synthetase

tRNA aminoacylation for protein translation lysyl-tRNA aminoacylation tRNA processing diadenosine tetraphosphate biosynthetic process viral process translation

Process(es)

tRNA binding lysine-tRNA ligase activity protein binding ATP binding amino acid binding metal ion binding nucleotide binding nucleic acid binding aminoacyl-tRNA ligase activity ligase activity