Invitrogen

KCNN4 Polyclonal Antibody

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

KCNN4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of formalin-fixed paraffin-embedded human pancreas using a KCNN4/KCa3.1 polyclonal antibody (Product # PA5-33875). Heat-induced antigen retrieval was performed prior to staining.

KCNN4 Antibody in Western Blot (WB) — Western blot analysis of KCNN4/KCa3.1/SK4 was performed by loading whole cell lysates from mouse kidney mCCDcl1 cells onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to PVDF membrane using the G2 Fast Blotter (Product # 62288) and blocked with 3% non-fat milk for at least 1 hour at room temperature. SK4 was detected using a SK4 polyclonal antibody (Product # PA5-33875) at a dilution of 1:2000 in blocking buffer overnight at 4°C on a rocking platform, followed by a goat anti-rabbit IgG-HRP secondary antibody (Product # A16104) at a dilution of 1:2,000 for at least 1 hour at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34078) and the myECL Imager (Product # 62236). Note: Data courtesy of our Innovators Program.

KCNN4 Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Fig 6 Differential expression of ion channels between principal cells (PC) and intercalated cells (IC) in the mouse kidney CCD. AQP2 staining was used to identify PC, the AQP2-positive cells, and IC, the APQ2-negative cells. Bar graphs give the mean +- SEM for the normalized intensities for each KCa channel for both PC and IC. The number of PC and IC cells analyzed, n, is indicated on the bar graphs. The images of CCD show representative immunofluorescence examples for each KCa channel and AQP2 (40X) where one or two PC (AQP2-positive) and IC (AQP2-negative) cells are labelled as ""PC"" or ""IC."" The outer border of the tubule in each image is indicated by the dashed white line (indicating the basal side or anti-luminal side of tubular cells). ""L"" identifies the tubular lumen. The immunofluorescence intensity was determined for each KCa channel using ImageJ and normalized to the intensity levels of AQP2 expression in PC (see Methods ). Each example gives staining for AQP2 (red), the KCa channel (green), and the merged image (AQP2, KCa channel) that includes DAPI staining to identify nuclei. A. Expression of SK1 in PC and IC showing dominant relative expression in IC over PC (P<0.001). SK1 expression was apparent within the cytosol and along the luminal border of both PC and IC as apparent in the representative images. Light staining along the basolateral border is also apparent in some cells. B. Expression of SK3 in PC and IC showing relatively more dominant expression in

KCNN4 Antibody in Immunohistochemistry (IHC) — Figure 3 Kcnn4 expression is decreased in the necrotic bones of IV BP-induced advanced-stage MRONJ patients. ( a ) High-magnification images (400x) of the immunohistochemistry analysis of Kcnn4 expression in the specimens of the two patient groups as indicated. Scale bars = 50 mum. Statistical analysis of the number of Kcnn4-positive cells ( b ), via the Mann-Whitney U test ( n = 20 per group). deg marks statistical outliers. For detailed data, see Table 2 . * p < 0.001.

Product Details

PA5-33875

Applications	Tested Dilution	Publications
Western Blot (WB)	1:2,000	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	10 µg/mL	-
Immunohistochemistry (IHC)	-	View 2 publications 2 publications
Immunohistochemistry (Frozen) (IHC (F))	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Bovine, Human, Mouse
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic 14 amino acid peptide from N-Terminus of human KCNN4. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.71 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS
Contains	0.1% sodium azide
Storage conditions	Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
RRID	AB_2551244

Product Specific Information

Percent identity with other species by BLAST analysis: Human, Gorilla, Bovine (100%) Marmoset, Panda, Dog, Rabbit, Pig (93%) Mouse, Rat, Guinea pig (86%).

Target Information

The protein encoded by this gene is part of a potentially heterotetrameric voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization, which promotes calcium influx. The encoded protein may be part of the predominant calcium-activated potassium channel in T-lymphocytes. This gene is similar to other KCNN family potassium channel genes, but it differs enough to possibly be considered as part of a new subfamily.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Comparison of the Effect of Oral Versus Intravenous Bisphosphonate Administration on Osteoclastogenesis in Advanced-Stage Medication-Related Osteonecrosis of the Jaw Patients.

Journal of clinical medicine

Kim HW,Lee MW,Lee JH,Kim MY

Published figure using KCNN4 polyclonal antibody (Product # PA5-33875) in Immunohistochemistry

Sun Jul 04 00:00:00 EDT 2021

Caveolae facilitate TRPV4-mediated Ca²⁺ signaling and the hierarchical activation of Ca²⁺-activated K⁺ channels in K⁺-secreting renal collecting duct cells.

American journal of physiology. Renal physiology

Li Y,Hu H,O'Neil RG

Published figure using KCNN4 polyclonal antibody (Product # PA5-33875) in Western Blot

Sat Dec 01 00:00:00 EST 2018

Expression of a Diverse Array of Ca2+-Activated K+ Channels (SK1/3, IK1, BK) that Functionally Couple to the Mechanosensitive TRPV4 Channel in the Collecting Duct System of Kidney.

PloS one

Li Y,Hu H,Butterworth MB,Tian JB,Zhu MX,O'Neil RG

PA5-33875 was used in immunohistochemistry - frozen section and western blot to utilize the mechanosensitive TRPV4 channel in the collecting duct system of kidney that is coupled to expression of a diverse array of Ca2+-activated K+ channels (SK1/3, IK1,

Wed Jul 26 00:00:00 EDT 2017

Expression of a Diverse Array of Ca2+-Activated K+ Channels (SK1/3, IK1, BK) that Functionally Couple to the Mechanosensitive TRPV4 Channel in the Collecting Duct System of Kidney.

PloS one

Li Y,Hu H,Butterworth MB,Tian JB,Zhu MX,O'Neil RG

Wed Jul 26 00:00:00 EDT 2017

Human neuronal changes in brain edema and increased intracranial pressure.

Acta neuropathologica communications

Faragó N,Kocsis ÁK,Braskó C,Lovas S,Rózsa M,Baka J,Kovács B,Mikite K,Szemenyei V,Molnár G,Ozsvár A,Oláh G,Piszár I,Zvara Á,Patócs A,Barzó P,Puskás LG,Tamás G

PA533875 was used in immunohistochemistry to measure mRNA expression and functional changes altered in response to edema and increased intracranial pressure in human neurons

Thu Aug 04 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: HIK1; IK1; IKCa; IKCa1; intermediate conductance calcium-activated potassium channel protein 1; Intermediate conductance calcium-activated potassium channel protein 4; KCa3.1; KCa4; KCNN 4; potassium channel, calcium activated intermediate/small conductance subfamily N alpha, member 4; potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4; putative erythrocyte intermediate conductance calcium-activated potassium Gardos channel; Putative Gardos channel; SK4; SKCa 4; SKCa4; small conductance calcium-activated potassium channel 4

Gene Aliases: DHS2; hIKCa1; hKCa4; hSK4; IK; IK1; IKCA1; KCa3.1; KCA4; KCNN4; mIKCa1; SK4; SKCas

UniProt ID: (Human) O15554, (Mouse) O89109

Entrez Gene ID: (Human) 3783, (Bovine) 534591, (Mouse) 16534

Molecular Function: voltage-gated ion channel