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CD29 (Integrin beta 1) Monoclonal Antibody (eBioHMb1-1 (HMb1-1)), NovaFluor™ Red 700, eBioscience™

View all (138) Integrin beta 1 (CD29) antibodies

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Product Details

M048T03R03-A

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

0.2 µg/test
-
Product Specifications

Species Reactivity

Mouse, Rat

Host/Isotype

Armenian hamster / IgG

Class

Monoclonal

Type

Antibody

Clone

eBioHMb1-1 (HMb1-1)

Conjugate

NovaFluor™ Red 700 NovaFluor™ Red 700 NovaFluor™ Red 700

Excitation/Emission Max

637/701 nm View spectra spectra

Form

Liquid

Concentration

0.1 mg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

RRID

AB_3099196

Product Specific Information

Description
The eBioHMb1-1 monoclonal antibody reacts with mouse and rat CD29 (integrin beta 1), a 110-120 kDa member of the beta integrin family expressed by leukocytes, endothelial, smooth muscle and epithelial cells.

This product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer.

Applications Tested
This eBioHMb1-1 (HMb1-1) antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.2 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Master mixes
• Master mixes of NFs should be made at 2-8 °C and may be made up to 4 hours ahead of time.
• We do not recommend storing master mixes containing NovaFluor conjugates overnight or longer.

Whole Blood compatibility
• When utilizing whole blood (as opposed to density-gradient-purified PBMC), we recommend lysing red blood cells in bulk prior to staining with NovaFluor conjugates.
• See the Bulk Lysis of Human Whole Blood protocol here.
• Staining of whole blood with NovaFluor conjugates followed by lysis of red blood cells may result in higher-than-expected background staining.

Viability dye compatibility
• NovaFluor dyes are not compatible with DNA intercalating viability dyes.
• Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes.

CellBlox Plus Blocking Buffer
• This NovaFluor conjugate comes with CellBlox Plus Blocking Buffer (for 25 µg or 25 test products, Cat. No. C001T02F01; for 100 µg or 100 test, Cat. No. C001T03F01), essential for optimal staining.
• Use CellBlox Plus Blocking Buffer in all experiments with NovaFluor conjugates.
• Add 5 µL per sample to antibody cocktails/master mixes (regardless of how many Novafluor-conjugated antibodies are present) before combining with cells.
• CellBlox Plus Blocking Buffer is compatible with either Super Bright Complete Blocking Buffer or Brilliant Stain Buffer and can be used in antibody cocktails/master mixes with those reagents.
• For single-color controls, use 5 µL of CellBlox Plus Blocking Buffer per 100 µL of cell sample (10^3 to 10^8 cells).

NovaFluor conjugates are based on Phiton technology utilizing novel fluorophore-containing nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts. Learn more

Excitation: 637 nm; Emission: 701 nm; Laser: 633-640 nm (Red) Laser

Target Information

CD29, also known as ITGB1 or Integrin Subunit Beta 1, is a 110 kDa cell surface glycoprotein widely expressed by various cells, including all leukocytes. It forms non-covalently linked heterodimers with at least six different alpha integrins (CD49a-f), creating VLA-1 through VLA-6 complexes, as well as with CD51. These alpha-beta integrin heterodimers mediate a range of cellular responses, including adhesion, trafficking, proliferation, and differentiation. CD29-containing integrins bind to extracellular matrix proteins such as collagen, laminin, fibronectin, and vitronectin. Some integrins also interact with cellular receptors like VCAM-1 and MadCAM-1. CD29 plays a crucial role in immune cell trafficking from blood to tissue, particularly during leukocyte emigration and extravascular migration. It is involved in regulating tissue damage and disease symptoms related to inflammatory bowel disease. In the presence of anti-tumor drugs, decreased expression of ITGB1 correlates with multidrug resistance in tumor cells. ITGB1-dependent mechanisms enhance cytokine secretion in human airway smooth muscle in response to IL-1beta, facilitated by fibronectin and type I collagen. More than eight beta subunits with numerous splice variant isoforms have been identified in mammals, including two major forms: beta1A and beta1D. Beta1A is present in all tissues except cardiac and skeletal muscle, which express the beta1D variant.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

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Bioinformatics

Protein Aliases: beta OL; beta oligodendroglia; collagen alpha-1(I) chain-like; fibronectin receptor subunit beta; integrin beta 1 (fibronectin receptor beta); Integrin beta1; integrin VLA-4 subunit beta; ITBG1D; unnamed protein product; VLA-4 subunit beta

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Gene Aliases: 4633401G24Rik; CD29; Fnrb; Gm9863; gpIIa

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UniProt ID: (Rat) P49134, (Mouse) P09055

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Entrez Gene ID: (Rat) 24511, (Mouse) 16412

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Function(s)
magnesium ion binding fibronectin binding protease binding actin binding obsolete cell adhesion receptor activity receptor binding integrin binding calcium ion binding protein binding collagen binding kinase binding protein kinase binding protein domain specific binding C-X3-C chemokine binding signaling receptor activity laminin binding macromolecular complex binding protein heterodimerization activity cell adhesion molecule binding alpha-actinin binding collagen binding involved in cell-matrix adhesion integrin binding involved in cell-matrix adhesion protein tyrosine kinase binding
Process(es)
G1/S transition of mitotic cell cycle establishment of mitotic spindle orientation angiogenesis in utero embryonic development cell fate specification tissue homeostasis cell migration involved in sprouting angiogenesis endocytosis phagocytosis cell adhesion leukocyte cell-cell adhesion cell-matrix adhesion calcium-independent cell-matrix adhesion transforming growth factor beta receptor signaling pathway integrin-mediated signaling pathway myoblast fusion regulation of G-protein coupled receptor protein signaling pathway positive regulation of cell proliferation negative regulation of cell proliferation germ cell migration visual learning response to radiation response to xenobiotic stimulus tissue development regulation of collagen catabolic process positive regulation of fibroblast migration positive regulation of cell-substrate adhesion positive regulation of neuron projection development response to activity response to muscle activity cell migration formation of radial glial scaffolds CD40 signaling pathway cell projection organization lamellipodium assembly positive regulation of cell migration neuron projection development negative regulation of cell projection organization cell-substrate adhesion receptor internalization response to nutrient levels protein transport within lipid bilayer cell adhesion mediated by integrin cell-cell adhesion mediated by integrin heterotypic cell-cell adhesion response to gonadotropin negative regulation of Rho protein signal transduction regulation of cell proliferation positive regulation of apoptotic process negative regulation of apoptotic process stress fiber assembly positive regulation of GTPase activity sarcomere organization myoblast differentiation negative regulation of cell differentiation negative regulation of neuron differentiation positive regulation of neuron differentiation positive regulation of fibroblast growth factor receptor signaling pathway positive regulation of angiogenesis positive regulation of cell adhesion positive regulation of endocytosis negative regulation of vasoconstriction mesodermal cell differentiation axon extension cardiac muscle tissue development dendrite morphogenesis positive regulation of peptidyl-tyrosine phosphorylation regulation of developmental process modulation of synaptic transmission leukocyte tethering or rolling positive regulation of transport regulation of cell cycle positive regulation of protein kinase B signaling positive regulation of glutamate uptake involved in transmission of nerve impulse cardiac muscle cell differentiation maternal process involved in female pregnancy bicellular tight junction assembly cellular response to mechanical stimulus cellular response to vitamin D cellular response to low-density lipoprotein particle stimulus cellular response to ionizing radiation response to transforming growth factor beta basement membrane organization positive regulation of wound healing establishment of Sertoli cell barrier cell-cell adhesion maintenance of postsynaptic specialization structure synaptic membrane adhesion to extracellular matrix regulation of spontaneous synaptic transmission regulation of postsynaptic neurotransmitter receptor diffusion trapping reactive gliosis positive regulation of vascular endothelial growth factor signaling pathway positive regulation of protein localization to plasma membrane regulation of synapse pruning negative regulation of anoikis regulation of neurotransmitter uptake
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