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Invitrogen
SSEA3 Monoclonal Antibody (MC-631), DyLight™ 650
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FIGURE: 1 / 2
SSEA3 Antibody (MA1-020-D650) in ICC/IF
Immunofluorescent analysis of SSEA-3 (red) showing membrane staining of NCCIT cells (right panel) compared to a negative HeLa control (left panel). The cells were fixed with formalin for 15 minutes, washed, and then blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a DyLight 650-conjugated SSEA-3 monoclonal antibody (Product # MA1-020-D650) in 3% BSA-PBS at a dilution of 1:20 and incubated for 1 hour at 37C in the dark. Nuclei (blue) were stained with DAPI. Images were taken at 60X magnification.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-020-D650
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rat
/ IgM
Class
Monoclonal
Type
Antibody
Clone
MC-631
Immunogen
Fischer rat immunized with 4-8 cell stage mouse embryos
Conjugate
DyLight™ 650
DyLight™ 650
DyLight™ 650
Excitation/Emission Max
651/673 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography - MBP
Storage buffer
PBS with proprietary stabilizer
Contains
0.02% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-020-D650 has been successfully used in ICC/IF and flow cytometry applications on human samples.
Target Information
SSEA-3 (stage-specific embryonic antigen 3) is a cell surface antigen, expressed along with SSEA-4, TRA-1-60 and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells, and induced pluripotent stem cells (iPS). In human cells, these surface markers are down-regulated during the differentiation process, while SSEA1 is absent in undifferentiated human stem cells and upregulated on the cell surface after retinoic acid mediated differentiation. Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: SSEA-3; stage-specific embryonic antigen 3
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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