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Invitrogen
SSEA4 Monoclonal Antibody (MC-813-70), DyLight™ 488
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FIGURE: 1 / 5
SSEA4 Antibody (MA1-021-D488) in ICC/IF
Immunofluorescent analysis of SSEA-4 (green) showing membrane staining of NCCIT cells (right panel) compared to negative HeLa cell control (left panel). The cells were fixed with formalin for 15 minutes, washed, and then blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a DyLight 488-conjugated SSEA-4 monoclonal antibody (Product # MA1-021-D488) in 3% BSA-PBS at a dilution of 1:150 and incubated for 1 hour at 37C in the dark. F-Actin (left panel, red) was stained with DyLight 554 Phalloidin (Product # 21834... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-021-D488
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG3
Class
Monoclonal
Type
Antibody
Clone
MC-813-70
Immunogen
human embryonal carcinoma cell line 2102Ep
Conjugate
DyLight™ 488
DyLight™ 488
DyLight™ 488
Excitation/Emission Max
492/519 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS with proprietary stabilizer
Contains
0.02% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-021-D488 has been successfully used in ICC/IF and flow cytometry applications on human samples.
Target Information
SSEA-4 (Stage-specific embryonic antigen 4) is a cell surface antigen, expressed along with SSEA-3, TRA-1-60 and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). In human cells, these surface markers are down-regulated during the differentiation process, while SSEA-1 is absent in undifferentiated human stem cells and upregulated on the cell surface after retinoic acid mediated differentiation. Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: SSEA-4; stage-specific embryonic antigen 4
Disclaimer
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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