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Invitrogen
SSEA4 Monoclonal Antibody (MC-813-70), DyLight™ 550
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FIGURE: 1 / 2
SSEA4 Antibody (MA1-021-D550) in ICC/IF
Immunofluorescent analysis of SSEA-4 (red) showing membrane staining of NCCIT cells (right panel) compared to negative HeLa cell control (left panel). The cells were fixed with formalin for 15 minutes, washed, and then blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a DyLight 550-conjugated SSEA-4 monoclonal antibody (Product # MA1-021-D550) in 3% BSA-PBS at a dilution of 1:100 and incubated for 1 hour at 37C in the dark. Nuclei (both panels, blue) were stained with DAPI. Images were taken at 60X magnific... View More
Product Details
MA1-021-D550
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
C. elegans
Host/Isotype
Mouse
/ IgG3
Class
Monoclonal
Type
Antibody
Clone
MC-813-70
Immunogen
human embryonal carcinoma cell line 2102Ep
Conjugate
Excitation/Emission Max
556/570 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS with proprietary stabilizer
Contains
0.02% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-021-D550 has been successfully used in ICC/IF and flow cytometry applications on human samples.
Target Information
SSEA-4 (Stage-specific embryonic antigen 4) is a cell surface antigen, expressed along with SSEA-3, TRA-1-60 and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). In human cells, these surface markers are down-regulated during the differentiation process, while SSEA-1 is absent in undifferentiated human stem cells and upregulated on the cell surface after retinoic acid mediated differentiation. Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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