Invitrogen

CD18 Monoclonal Antibody (M18/2)

Name: CD18 Monoclonal Antibody (M18\/2)
Brand: Invitrogen
SKU: MA1-10122

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1 Published Figure

1 Reference

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Additional Information:

FIGURE: 1 / 4

CD18 Antibody (MA1-10122) in Flow

Separation of murine myeloid cells stained using anti-mouse CD18 (M18/2) purified Monoclonal antibody (Product # MA1-10122) (concentration in sample 16 µg/mL, DAR APC, red-filled) from murine myeloid cells unstained by primary antibody (DAR APC, black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension.

CD18 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

CD18 Antibody — Fig. 3. Inhibition of VEGF-induced vascular leakage by anti-beta2 integrin antibody. A , levels of fibronectin in the retina measured by ELISA. Anti-VEGF, VEGF plus anti-VEGF antibody ( n = 4). B , levels of beta2 integrin in the retina measured by Western blotting analysis ( n = 3). C , qualitative assessment of retinal vascular integrity demonstrated by the leakage of FITC-dextran after treatments of PBS ( control ), VEGF ( VEGF ), and VEGF plus anti-beta2 integrin antibody ( anti -beta 2 integrin ). D , quantitative analysis of relative fluorescence intensity after treatments of VEGF ( VEGF ) and VEGF plus anti-beta2 integrin antibody ( anti -beta 2 integrin ) ( n = 4). E , levels of phosphorylated FAK normalized by the total amount of FAK ( p-FAK/total FAK ) in confluent HRMECs on FN1-coated dish at 5 min after treatments of VEGF ( VEGF ) and VEGF plus anti-beta2 integrin antibody ( Anti -beta 2 integrin ) ( n = 4). F , actin cytoskeleton arrangement after treatments of PBS ( Control ), VEGF ( VEGF ), and VEGF plus anti-beta2 integrin antibody ( Anti -beta 2 integrin ) demonstrated by immunofluorescence staining of F-actin ( green ). Nucleus was identified with DAPI staining. NS , p > 0.05; *, p < 0.05; **, p < 0.01 using Kruskal-Wallis test with post hoc Dunn's multiple comparison test. {TM}

Immunogen image

Immunogen Image CD18 Monoclonal Antibody (M18/2)

Product Details

MA1-10122

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Flow Cytometry (Flow)

1 µg/mL

Immunoprecipitation (IP)

Assay-Dependent

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

M18/2

Immunogen

Murine cytotoxic T cell glycoproteins

3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

100 µg

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4°C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11152311

Product Specific Information

This antibody recognizes an extracellular epitope of CD18 antigen (integrin beta2 subunit; beta2 integrin), a 95 kDa type I transmembrane protein expressed on all leukocytes.

This antibody will not cross-react with canine.

Target Information

CD18, also known as the integrin beta 2 subunit, is a 90-95 kDa type I transmembrane protein expressed on all leukocytes. It heterodimerizes with the alpha chains of CD11a, CD11b, CD11c, and CD11d to form various leukocyte (beta2) integrins, which are crucial for cellular adhesion and signaling. The CD18/CD11a complex, known as lymphocyte function-associated antigen-1 (LFA-1), plays a significant role in cellular adhesion and inflammatory reactions. CD18 also forms heterodimers with CD11b (Mac-1, CR3), CD11c, and CD11d, contributing to the processes of cell adhesion and cell-surface mediated signaling. These integrins are essential for proper leukocyte migration and mediating intercellular contacts. The absence of CD18 leads to leukocyte adhesion deficiency type I (LAD1), a condition characterized by impaired leukocyte migration. Severe reduction in CD18 expression can result in the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin, which can mediate leukotoxin-induced cytolysis. Defects in the CD18 gene are the cause of LAD1, and two transcript variants encoding the CD18 protein have been identified. The integrin-mediated responses are often a composite of the functions of its individual subunits, highlighting the importance of CD18 in immune function and disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CD18; Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; Complement receptor C3 subunit beta; Integrin beta-2; lymphocyte function associated antigen 1; Mac-1 beta; macrophage antigen-1 beta

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Gene Aliases: 2E6; AI528527; Cd18; Itgb2; LAD; LCAMB; Lfa1; MF17

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UniProt ID: (Mouse) P11835

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Entrez Gene ID: (Mouse) 16414

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Function(s)

glycoprotein binding receptor activity protein binding protein kinase binding ICAM-3 receptor activity protein complex binding metal ion binding protein heterodimerization activity cell adhesion molecule binding integrin

Process(es)

leukocyte migration involved in inflammatory response cell adhesion leukocyte cell-cell adhesion cell-matrix adhesion signal transduction integrin-mediated signaling pathway single organismal cell-cell adhesion natural killer cell activation neutrophil chemotaxis heterotypic cell-cell adhesion receptor clustering endothelial cell migration cellular extravasation positive regulation of nitric oxide biosynthetic process positive regulation of angiogenesis regulation of peptidyl-tyrosine phosphorylation activated T cell proliferation positive regulation of NF-kappaB transcription factor activity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD18 Monoclonal Antibody (M18/2)

CD18 Antibody (MA1-10122) in Flow

Product Details

MA1-10122

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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