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Invitrogen
CD18 Monoclonal Antibody (M18/2), FITC
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-10124
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
M18/2
Immunogen
Murine cytotoxic T cell glycoproteins
Conjugate
FITC
FITC
FITC
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Size-exclusion chromatography
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody recognizes an extracellular epitope of CD18 antigen (integrin beta2 subunit; beta2 integrin), a 95 kDa type I transmembrane protein expressed on all leukocytes.
This antibody will not cross-react with canine.
Target Information
CD18, also known as the integrin beta 2 subunit, is a 90-95 kDa type I transmembrane protein expressed on all leukocytes. It heterodimerizes with the alpha chains of CD11a, CD11b, CD11c, and CD11d to form various leukocyte (beta2) integrins, which are crucial for cellular adhesion and signaling. The CD18/CD11a complex, known as lymphocyte function-associated antigen-1 (LFA-1), plays a significant role in cellular adhesion and inflammatory reactions. CD18 also forms heterodimers with CD11b (Mac-1, CR3), CD11c, and CD11d, contributing to the processes of cell adhesion and cell-surface mediated signaling. These integrins are essential for proper leukocyte migration and mediating intercellular contacts. The absence of CD18 leads to leukocyte adhesion deficiency type I (LAD1), a condition characterized by impaired leukocyte migration. Severe reduction in CD18 expression can result in the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin, which can mediate leukotoxin-induced cytolysis. Defects in the CD18 gene are the cause of LAD1, and two transcript variants encoding the CD18 protein have been identified. The integrin-mediated responses are often a composite of the functions of its individual subunits, highlighting the importance of CD18 in immune function and disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; complement receptor C3 subunit beta; lymphocyte function associated antigen 1; Mac-1 beta; macrophage antigen-1 beta; unnamed protein product
Gene Aliases: 2E6; Cd18; LAD; LCAMB; Lfa1; MF17
UniProt ID: (Mouse) P11835
Entrez Gene ID: (Mouse) 16414
leukocyte migration involved in inflammatory response
receptor-mediated endocytosis
phagocytosis
phagocytosis, engulfment
cell adhesion
neuron cell-cell adhesion
leukocyte cell-cell adhesion
cell-matrix adhesion
integrin-mediated signaling pathway
I-kappaB kinase/NF-kappaB signaling
neutrophil chemotaxis
receptor internalization
positive regulation of superoxide anion generation
cell adhesion mediated by integrin
cell-cell adhesion mediated by integrin
heterotypic cell-cell adhesion
receptor clustering
positive regulation of neutrophil degranulation
endothelial cell migration
cellular extravasation
leukocyte activation
positive regulation of nitric oxide biosynthetic process
positive regulation of angiogenesis
regulation of peptidyl-tyrosine phosphorylation
activated T cell proliferation
leukocyte adhesion to vascular endothelial cell
cellular response to low-density lipoprotein particle stimulus
beta-amyloid clearance
cell-cell adhesion
positive regulation of leukocyte adhesion to vascular endothelial cell
neutrophil migration
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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