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Invitrogen
SSEA5 Monoclonal Antibody (8E11), PE
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FIGURE: 1 / 1
SSEA5 Antibody (MA1-144-PE) in Flow
Flow cytometry analysis of SSEA-5 in NCCIT (red filled histogram) and HeLa cells (red unfilled histogram) compared to unstained cells (blue histogram). Positive staining is observed on NCCIT cells when compared to no antibody control (middle panel) and to SSEA-5-negative HeLa cells (right panel). Cells were fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with 2% BSA-PBS for 30 minutes at room temperature and incubated with a PE-conjugated SSEA-5 monoclonal antibody (Product # MA1-144-PE) in 2% BSA-PBS at a dilution o... View More
Product Details
MA1-144-PE
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
8E11
Immunogen
Undifferentiated H9 human embryonic stem cells
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunofluorescence and flow cytometric analysis of MA1-144-PE shows positive staining of SSEA-5 in human embryonal carcinoma NCCIT cells.
Target Information
Stage-Specific Embryonic Antigen-5 (SSEA-5) is an oligosaccharide that can be part of glycosphingolipids and glycoproteins. It contains the motif Fuc1-2Gal-beta1-3GlcNAc-beta, which is characteristic of H-1 antigens. SSEA-5 is found in the inner cell mass of the human blastocyst during embryogenesis, on undifferentiated human embryonic stem cells and on the surface of induced pluripotent cells. SSEA-5 was found to proceed the loss of other known stem cell markers and the combination of SSEA-5 with either CD9 and CD90 or CD50 and CD200 was superior in identifying and eliminating rare undifferentiated human ES cells from differentiated cell populations than the use of other established stem cell markers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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