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Invitrogen
ATG9A Monoclonal Antibody (14F2 8B1)
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FIGURE: 1 / 2
ATG9A Antibody (MA1-149) in ICC/IF
Immunofluorescent analysis of ATG9 (green) accumulation in HeLa cells in response to serum starvation (0.1% FCS, 16 hours). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with an ATG9 monoclonal antibody (Product # MA1-149) at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-Armenian Hamster IgG secondary antibody. Images were taken o... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-149
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Armenian hamster
/ IgG
Class
Monoclonal
Type
Antibody
Clone
14F2 8B1
Immunogen
peptide sequence HPEPVPEEGSEDELPPQVHK of human ATG9A C-terminus
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS with 1mg/mL BSA, 30% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-149 was produced in Armenian hamster and detects ATG9 in human, rat and mouse samples. MA1-149 has been successfully used in Western Blot, Immunofluorescence, and ELISA procedures. Western Blot analysis with MA1-149 shows the detection of a double band at ~85-95 kDa in human ATG9 overerexpression lysates. MA1-149 also detects additional unknown bands at ~40 and ~60 kDa. In Immunofluorescence applications, MA1-149 shows accumulation and redestribution of ATG9 in response to starvation-induced autophagosome assembly.
Target Information
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). Apg9 plays a direct role in the formation of the cytoplasm to vacuole targeting and autophagic vesicles, possibly serving as a marker for a specialized compartment essential for these vesicle-mediated alternative targeting pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Apg9; APG9 autophagy 9-like 1; APG9-like 1; Apg911; APG9A; ATG 9A; ATG9 autophagy related 9 homolog A; autophagy 9-like 1 protein; autophagy protein 9; autophagy-related 9-like 1; FLJ22169; unnamed protein product
Gene Aliases: APG9L1; Atg9; Atg9l1; mATG9; MGD3208; RGD1310450
UniProt ID: (Human) Q7Z3C6, (Rat) Q5FWU3, (Mouse) Q68FE2
Entrez Gene ID: (Human) 79065, (Rat) 363254, (Mouse) 245860
autophagosome assembly
mitophagy
autophagy
phospholipid scrambling
response to nutrient levels
negative regulation of interferon-beta production
protein localization to Golgi apparatus
protein localization to pre-autophagosomal structure
piecemeal microautophagy of nucleus
innate immune response
bone morphogenesis
reticulophagy
programmed necrotic cell death
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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