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Invitrogen
ATG9A Monoclonal Antibody (14F2 8B1), Alexa Fluor™ 555
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FIGURE: 1 / 1
ATG9A Antibody (MA1-149-A555) in ICC/IF
Immunofluorescent analysis of ATG9A (red) in 0.1% serum starved (left) and non-serum-starved (right) U2OS cells. The cells were fixed with 4% Paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were stained with a ATG9A mouse monoclonal antibody (Product # MA1-149-A555) at a concentration of 5 µg/mL in blocking buffer for 1 hour at room temperature. Nuclei (blue) were stained with Hoechst 33342 (Product # 62249). Images were taken on a Therm... View More
Product Details
MA1-149-A555
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Armenian hamster
/ IgG
Class
Monoclonal
Type
Antibody
Clone
14F2 8B1
Immunogen
peptide sequence HPEPVPEEGSEDELPPQVHK of human ATG9A C-terminus
Conjugate
Excitation/Emission Max
553/568 nm
View spectra
Form
Liquid
Concentration
1.0 mg/mL
Amount
50 µg
Purification
Protein A
Storage buffer
PBS with 1.0mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-149A555 was produced in Armenian hamster and detects ATG9 in human, rat and mouse samples. In Immunofluorescence applications, MA1-149A555 shows accumulation and redestribution of ATG9 in response to starvation-induced autophagosome assembly.
Target Information
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). Apg9 plays a direct role in the formation of the cytoplasm to vacuole targeting and autophagic vesicles, possibly serving as a marker for a specialized compartment essential for these vesicle-mediated alternative targeting pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Apg9; APG9 autophagy 9-like 1; APG9-like 1; Apg911; APG9A; ATG 9A; ATG9 autophagy related 9 homolog A; autophagy 9-like 1 protein; autophagy protein 9; autophagy-related 9-like 1; autophagy-related 9A; Autophagy-related protein 9A; FLJ22169; mATG9; unnamed protein product
Gene Aliases: APG9L1; Atg9; ATG9A; Atg9l1; AU019532; mATG9; MGD3208; RGD1310450
UniProt ID: (Human) Q7Z3C6, (Mouse) Q68FE2, (Rat) Q5FWU3
Entrez Gene ID: (Human) 79065, (Mouse) 245860, (Rat) 363254
autophagosome assembly
mitophagy
transport
autophagy
protein transport
negative regulation of interferon-beta production
protein localization to Golgi apparatus
protein localization to pre-autophagosomal structure
piecemeal microautophagy of nucleus
late nucleophagy
innate immune response
mitophagy
lipid transport
negative regulation of macrophage cytokine production
phospholipid scrambling
bone morphogenesis
reticulophagy
programmed necrotic cell death
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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