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Invitrogen
BUBR1 Monoclonal Antibody (8G1)
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FIGURE: 1 / 4
BUBR1 Antibody (MA1-16577) in WB
Western blot was performed using Anti-BUBR1 Monoclonal Antibody (8G1)(Product # MA1-16577) and a 120kDa band corresponding to BUBR1 was observed across cell lines and tissues tested. Whole cell extracts (30 µg lysate) of RD (Lane 1), SK-O-V3 (Lane 2), HeLa (Lane 3), MDA-MB-231 (Lane 4), MCF7 (Lane 5), PC-3 (Lane 6), Mouse Thymus (Lane 7), Rat Thymus (Lane 8),Mouse Liver (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Produc... View More
Product Details
MA1-16577
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
8G1
Immunogen
A portion of human BubR1 tagged at the N-terminus with pMAL (residues 1-350).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
0.1% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Suggested positive control: Hela whole cell extract, antigen standard for BUB1B (transient overexpression lysate).
Target Information
BUBR1 is a member of the Ser/Thr protein kinase family. It is a component of the mitotic checkpoint that delays anaphase until all chromosome are properly attached to the mitotic spindle. Compromised spindle checkpoints are thought to play an important role in genetic instability that predisposes cells to malignant transformations. Human cells contain two related checkpoint protein kinases, evolved from the BUB1 gene; hBUBR1 and hBUB1. They are important components of the spindle checkpoint Both kinases monitor mitotic kinetochore-microtubule interactions. hBUBR1 is essential for normal mitotic progression as it prevents cells from entering into anaphase too early. BUBR1 has been detected in human cancers. It has been identified as a novel binding partner of BCSG1 (Breast Cancer-Specific Gene 1), which has been suggested to accelerate the progression of breast cancer by compromising the mitotic checkpoint control through inactivation of BUBR1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: BUB1B, mitotic checkpoint serine/threonine kinase; BubR1; budding uninhibited by benzimidazoles 1 beta; budding uninhibited by benzimidazoles 1 homolog beta; hBub1; hBUBR1; MAD3/BUB1-related protein kinase; Mitotic checkpoint kinase MAD3L; Mitotic checkpoint serine/threonine-protein kinase BUB1 beta; Protein SSK1; similar to protein kinase; unnamed protein product
Gene Aliases: AU045529; Bub1A; BUB1B; BUB1beta; BUBR1; hBUBR1; MAD3L; MVA1; SSK1
UniProt ID: (Human) O60566, (Mouse) Q9Z1S0
Entrez Gene ID: (Human) 701, (Rat) 171576, (Mouse) 12236
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
protein binding
ATP binding
kinase activity
transferase activity
protein serine kinase activity
molecular_function
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
apoptotic process
metaphase/anaphase transition of mitotic cell cycle
mitotic spindle assembly checkpoint
regulation of sister chromatid segregation
cell division
meiotic sister chromatid cohesion, centromeric
protein localization to chromosome, centromeric region
protein phosphorylation
mitotic nuclear division
phosphorylation
protein localization to kinetochore
cell cycle
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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