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Invitrogen
PDI Monoclonal Antibody (RL90), Alexa Fluor™ 647
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FIGURE: 1 / 1
PDI Antibody (MA3-019-A647) in ICC/IF
Immunofluorescent analysis of PDI (red) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a PDI Monoclonal Antibody, AlexaFluor 647 conjugate (Product # MA3-019-A647) at a dilution of 2.5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images w... View More
Product Details
MA3-019-A647
Product Specifications
Species Reactivity
Hamster,
Human,
Mouse,
Pig,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
RL90
Immunogen
Purified rat PDI protein
Conjugate
Excitation/Emission Max
650/671 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
50 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA3019A647 detects protein disulphide-isomerase (PDI) from human, rat, porcine and mouse tissues as well as hamster cells.
The MA3019A647 antigen is purified rat PDI.
Target Information
Protein disulfide isomerase (PDI) is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, the protein seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. It may therefore cause structural modifications of exofacial proteins. Inside the cell, PDI seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, it functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, it facilitates aggregation (anti-chaperone activity). PDI may be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. It also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CaO19.12595; Cellular thyroid hormone-binding protein; collagen prolyl 4-hydroxylase beta; Endoplasmic reticulum resident protein 59; Eps1p; ER protein 59; glutathione-insulin transhydrogenase; p55; p58; PDI; PDI1P; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide; prolyl 4-hydroxylase beta polypeptide; Prolyl 4-hydroxylase subunit beta; prolyl 4-hydroxylase, beta polypeptide; protein disulfide isomerase; Protein disulfide isomerase (Prolyl 4-hydroxylase, beta polypeptide); protein disulfide isomerase family A, member 1; protein disulfide isomerase-associated 1; protein disulfide isomerase/oxidoreductase; Protein disulfide-isomerase; protocollagen hydroxylase; testicular secretory protein Li 32; thyroid hormone binding protein precursor; thyroid hormone-binding protein p55; unnamed protein product
Gene Aliases: CLCRP1; DSI; ERBA2L; ERp59; GIT; I79_002399; P4HB; P4Hbeta; p58; PDI; PDIA1; PDIR; PHDB; PO4DB; PO4HB; PROHB; Thbp
UniProt ID: (Human) P07237, (Rat) P04785, (Mouse) P09103, (Chinese hamster) Q8R4U2
Entrez Gene ID: (Pig) 110255932, (Human) 5034, (Rat) 25506, (Mouse) 18453, (Chinese hamster) 100689433
RNA binding
protein disulfide isomerase activity
actin binding
procollagen-proline 4-dioxygenase activity
integrin binding
protein binding
protein disulfide oxidoreductase activity
isomerase activity
thiol oxidase activity
enzyme binding
macromolecular complex binding
protein heterodimerization activity
peptidyl-proline 4-dioxygenase activity
poly(A) RNA binding
chaperone
protein folding
ER to Golgi vesicle-mediated transport
peptidyl-proline hydroxylation to 4-hydroxy-L-proline
insulin processing
protein folding in endoplasmic reticulum
response to endoplasmic reticulum stress
interleukin-12-mediated signaling pathway
interleukin-23-mediated signaling pathway
positive regulation of cell adhesion
positive regulation of viral entry into host cell
cellular response to hypoxia
cellular response to interleukin-7
positive regulation of substrate adhesion-dependent cell spreading
regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
positive regulation of T cell migration
cell redox homeostasis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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