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Invitrogen
PDI Monoclonal Antibody (RL90)
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FIGURE: 1 / 78
PDI Antibody (MA3-019) in ICC/IF
Immunofluorescent analysis of Phalloidin (orange) and PDI (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a PDI monoclonal antibody (Product # MA3-019) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution ... View More
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.jpg?time=20220826?time=20220826 "PDI Antibody (MA3-019) in WB")
Product Details
MA3-019
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Hamster,
Human,
Mouse,
Pig,
Rat
Published species
Dog,
Hamster,
Human,
Mouse,
Non-human primate,
Rat,
Rhesus monkey
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
RL90
Immunogen
Purified rat PDI protein.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA3-019 detects protein disulphide-isomerase (PDI) from human, rat, porcine and mouse tissues as well as hamster cells.
MA3-019 has been successfully used in Western blot, immunofluorescence, immunohistochemical, flow cytometry, and immunoprecipitation procedures. By Western blot, this antibody detects a protein at 59 kDa representing PDI from rat liver extract or a slightly higher protein at 61 kDa representing PDI from human liver extract. Immunohistochemical staining of PDI in rat intestine with MA3-019 yields a pattern consistent with cytoplasmic staining. In immunoprecipitation procedures MA3-019 has been shown to inhibit the activity of PDI in vitro. MA3-019 has also been found to inhibit disulfide bond reduction of the HIV protein, gp120, at the cell surface of CHO cells and human lymphoid cells.
In immunohistochemistry procedures, formalin-fixed paraffin-embedded tissue sections are recommended.
The MA3-019 antigen is purified rat PDI.
Target Information
Protein disulfide isomerase (PDI) is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, the protein seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. It may therefore cause structural modifications of exofacial proteins. Inside the cell, PDI seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, it functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, it facilitates aggregation (anti-chaperone activity). PDI may be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. It also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CaO19.12595; Cellular thyroid hormone-binding protein; collagen prolyl 4-hydroxylase beta; Endoplasmic reticulum resident protein 59; Eps1p; ER protein 59; glutathione-insulin transhydrogenase; p55; p58; PDI; PDI1P; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide; prolyl 4-hydroxylase beta polypeptide; Prolyl 4-hydroxylase subunit beta; prolyl 4-hydroxylase, beta polypeptide; protein disulfide isomerase; Protein disulfide isomerase (Prolyl 4-hydroxylase, beta polypeptide); protein disulfide isomerase family A, member 1; protein disulfide isomerase-associated 1; protein disulfide isomerase/oxidoreductase; Protein disulfide-isomerase; protocollagen hydroxylase; testicular secretory protein Li 32; thyroid hormone binding protein precursor; thyroid hormone-binding protein p55; unnamed protein product
Gene Aliases: CLCRP1; DSI; ERBA2L; ERp59; GIT; I79_002399; P4HB; P4Hbeta; p58; PDI; PDIA1; PDIR; PHDB; PO4DB; PO4HB; PROHB; Thbp
UniProt ID: (Human) P07237, (Rat) P04785, (Mouse) P09103, (Chinese hamster) Q8R4U2
Entrez Gene ID: (Pig) 110255932, (Human) 5034, (Rat) 25506, (Mouse) 18453, (Chinese hamster) 100689433
RNA binding
protein disulfide isomerase activity
actin binding
procollagen-proline 4-dioxygenase activity
integrin binding
protein binding
protein disulfide oxidoreductase activity
isomerase activity
thiol oxidase activity
enzyme binding
macromolecular complex binding
protein heterodimerization activity
peptidyl-proline 4-dioxygenase activity
poly(A) RNA binding
chaperone
protein folding
ER to Golgi vesicle-mediated transport
peptidyl-proline hydroxylation to 4-hydroxy-L-proline
insulin processing
protein folding in endoplasmic reticulum
response to endoplasmic reticulum stress
interleukin-12-mediated signaling pathway
interleukin-23-mediated signaling pathway
positive regulation of cell adhesion
positive regulation of viral entry into host cell
cellular response to hypoxia
cellular response to interleukin-7
positive regulation of substrate adhesion-dependent cell spreading
regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
positive regulation of T cell migration
cell redox homeostasis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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