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Invitrogen
Cytochrome C Monoclonal Antibody (6H2.B4), Alexa Fluor™ 555
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FIGURE: 1 / 4
Cytochrome C Antibody (MA3-38200-A555) in ICC/IF
Knockdown of Cytochrome C was achieved by transfecting HeLa cells with Cytochrome C specific siRNA (Silencer® select Product # S28898, S28897). Immunofluorescence analysis was performed on Untransfected HeLa cells (panel a-d), transfected with non-specific scrambled siRNA (panels e-h) and transfected with Cytochrome C specific siRNA (panel i-l). Cells were fixed, permeabilized, and labelled with Cytochrome ... View More
Product Details
MA3-38200-A555
Product Specifications
Species Reactivity
Fruit fly,
Human,
Mouse,
Rabbit,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
6H2.B4
Immunogen
Rat cytochrome C
Conjugate
Excitation/Emission Max
553/568 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
50 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody reacts with native Cytochrome C, but does not recognize denatured cytochrome C. The antibody recognizes a region in the vicinity of amino acid 62 of rat Cytochrome C.
Target Information
Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CG17903-PA; CG17903-PB; CRF; CYCS; Cyt-c-p-PA; Cyt-c-p-PB; Cytochrome c; cytochrome c DC4; Cytochrome C, expressed in somatic tissues; Cytochrome c, somatic; Cytochrome c-2; Cytochrome c-proximal; cytochrome-c-p; Cytochrome-c-proximal; hc1; lethal (2) k13905; RH17228p; unnamed protein product
Gene Aliases: CG17903; CYC; CYC2; CYCS; CYCSA; Cyct-c-p; Cyt C; cyt c-p; Cyt-c; Cyt-c-p; Cyt-c2; cyt.c; CytC; CytC-2; Cytc-p; CYTC2; cytochrome c; DC4; dCyt.C-p; DMc01; Dmel\CG17903; Dmel_CG17903; HCS; l(2)k13905; pDMc01; THC4
UniProt ID: (Human) P99999, (Mouse) P62897, (Rat) P62898, (Fruit fly) P84029
Entrez Gene ID: (Human) 54205, (Mouse) 13063, (Rat) 25309, (Fruit fly) 34996, (Rabbit) 100341175, (Rabbit) 100357840
mitochondrial electron transport, ubiquinol to cytochrome c
mitochondrial electron transport, cytochrome c to oxygen
apoptotic process
cellular respiration
apoptotic signaling pathway
intrinsic apoptotic signaling pathway
execution phase of apoptosis
apoptotic DNA fragmentation
activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c
hydrogen peroxide metabolic process
ATP synthesis coupled electron transport
oxidative phosphorylation
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
oxidation-reduction process
response to ischemia
response to oxidative stress
negative regulation of hydrogen peroxide biosynthetic process
glial cell apoptotic process
positive regulation of cellular respiration
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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