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Invitrogen
Cytochrome C Monoclonal Antibody (6H2.B4)
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FIGURE: 1 / 6
Cytochrome C Antibody (33-8200) in ICC/IF
Immunofluorescence analysis of Cytochrome C was performed using 70 % confluent log phase HeLa cells. The cells were fixed with 4 % paraformaldehyde for 10 minutes, permeabilized with 0.1 % Triton™ X-100 for 15 minutes, and blocked with 2 % BSA for 45 minutes at room temperature. The cells were labeled with Cytochrome C Monoclonal Antibody (6H2.B4) (Product # 33-8200, 1.66 µg/mL) in 0.1 % BSA, incubated at 4... View More
Product Details
33-8200
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Fruit fly,
Human,
Mouse,
Rabbit,
Rat
Published species
Chicken,
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
6H2.B4
Immunogen
Rat Cytochrome C.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody reacts with native Cytochrome C, but does not recognize denatured cytochrome C. The antibody recognizes a region in the vicinity of amino acid 62 of rat Cytochrome C.
Target Information
Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CG17903-PA; CG17903-PB; CRF; CYCS; Cyt-c-p-PA; Cyt-c-p-PB; Cytochrome c; cytochrome c DC4; Cytochrome C, expressed in somatic tissues; Cytochrome c, somatic; Cytochrome c-2; Cytochrome c-proximal; cytochrome-c-p; Cytochrome-c-proximal; hc1; lethal (2) k13905; RH17228p; unnamed protein product
Gene Aliases: CG17903; CYC; CYC2; CYCS; CYCSA; Cyct-c-p; Cyt C; cyt c-p; Cyt-c; Cyt-c-p; Cyt-c2; cyt.c; CytC; CytC-2; Cytc-p; CYTC2; cytochrome c; DC4; dCyt.C-p; DMc01; Dmel\CG17903; Dmel_CG17903; HCS; l(2)k13905; pDMc01; THC4
UniProt ID: (Human) P99999, (Mouse) P62897, (Rat) P62898, (Fruit fly) P84029
Entrez Gene ID: (Human) 54205, (Mouse) 13063, (Rat) 25309, (Fruit fly) 34996, (Rabbit) 100341175, (Rabbit) 100357840
mitochondrial electron transport, ubiquinol to cytochrome c
mitochondrial electron transport, cytochrome c to oxygen
apoptotic process
cellular respiration
apoptotic signaling pathway
intrinsic apoptotic signaling pathway
execution phase of apoptosis
apoptotic DNA fragmentation
activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c
hydrogen peroxide metabolic process
ATP synthesis coupled electron transport
oxidative phosphorylation
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
oxidation-reduction process
response to ischemia
response to oxidative stress
negative regulation of hydrogen peroxide biosynthetic process
glial cell apoptotic process
positive regulation of cellular respiration
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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