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Invitrogen
PCNA Monoclonal Antibody (PC10), Biotin
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FIGURE: 1 / 9
PCNA Antibody (MA5-11355) in WB
Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), MCF7 (Lane 2), Jurkat (Lane 3), HEK-293 (Lane 4), NIH/3T3 (Lane 5), MDA-MB-231 (Lane 6), A549 (Lane 7), SH-SY5Y (Lane 8) and Raji (Lane 9). The blot was probed with anti-PCNA Monoclonal Antibody (Product # MA5-11355, 1:500 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 34 kDa band corresponding to PCNA was ... View More
Product Details
MA5-11355
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Amphibian,
Cat,
Dog,
Guinea pig,
Human,
Mouse,
Non-human primate,
Pig,
Rabbit,
Rat,
Rhesus monkey,
Xenopus,
Yeast,
Zebrafish
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
PC10
Immunogen
Recombinant rat PCNA protein
Conjugate
Form
Liquid
Concentration
200 µg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Methanol fixation is required for immunofluorescence and flow cytometry staining. To decrease nonspecific staining for both applications, using a Biotin/Streptavidin blocker is recommended.
By WB, MA5-11355 detects a predominant band between 30-35kD, and fainter nonspecific bands at ~72kD and 130kD.
Target Information
PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. PCNA is found in the nucleus and is a cofactor of DNA polymerase delta, and acts as a homotrimer and helps increase the processing of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of PCNA have been described on chromosome 4 and on the X chromosome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cyclin; DNA polymerase delta auxiliary protein; DNA sliding clamp PCNA; HGCN8729; MGC8367; PCNA; Proliferating cell nuclear antigen; unnamed protein product
Gene Aliases: ATLD2; PCNA; Pcna/cyclin; PCNAR
UniProt ID: (Human) P12004, (Rat) P04961
Entrez Gene ID: (Human) 5111, (Rat) 25737
purine-specific mismatch base pair DNA N-glycosylase activity
DNA binding
chromatin binding
damaged DNA binding
protein binding
enzyme binding
estrogen receptor binding
DNA polymerase processivity factor activity
receptor tyrosine kinase binding
dinucleotide insertion or deletion binding
MutLalpha complex binding
histone acetyltransferase binding
identical protein binding
macromolecular complex binding
DNA polymerase binding
transcription factor binding
DNA polymerase processivity factor
negative regulation of transcription from RNA polymerase II promoter
DNA replication
leading strand elongation
regulation of DNA replication
DNA repair
base-excision repair, gap-filling
mismatch repair
chromatin organization
cellular response to DNA damage stimulus
response to oxidative stress
heart development
translesion synthesis
epithelial cell differentiation
replication fork processing
positive regulation of deoxyribonuclease activity
response to estradiol
response to lipid
cellular response to UV
estrous cycle
positive regulation of DNA repair
positive regulation of DNA replication
response to cadmium ion
cellular response to hydrogen peroxide
cellular response to xenobiotic stimulus
response to dexamethasone
liver regeneration
positive regulation of DNA-directed DNA polymerase activity
response to L-glutamate
mitotic telomere maintenance via semi-conservative replication
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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