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Invitrogen
PCNA Monoclonal Antibody (PC10)
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FIGURE: 1 / 35
PCNA Antibody (MA5-11358) in ICC/IF
Immunofluorescent analysis of PCNA (purple) in HeLa cells. The cells were fixed and permeabilized with ice-cold methanol for 15 minutes, and blocked with 1% Blocker BSA in PBS (Product # 37525) for 15 minutes at room temperature. Cells were stained with a PCNA monoclonal antibody (Product # MA5-11358) at a dilution of 1:50 in 1% Blocker BSA in PBS (right panel) or incubated in blocking buffer alone as a negative control (left panel) for 1 hour at room temperature, and then incubated with a DyLight 650-conjugated goat anti-mouse IgG seconda... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-11358
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Non-human primate,
Rat
Published species
Amphibian,
Avian,
Cat,
Dog,
Fish,
Guinea pig,
Human,
Mouse,
Non-human primate,
Pig,
Rabbit,
Rat,
Rhesus monkey,
Xenopus,
Yeast,
Zebrafish
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
PC10
Immunogen
Recombinant rat PCNA protein
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.02 mg/mL
Amount
10 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Methanol fixation is required for IF staining.
Target Information
PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. PCNA is found in the nucleus and is a cofactor of DNA polymerase delta, and acts as a homotrimer and helps increase the processing of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of PCNA have been described on chromosome 4 and on the X chromosome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cyclin; DNA polymerase delta auxiliary protein; HGCN8729; MGC8367; PCNA; unnamed protein product
Gene Aliases: ATLD2; Pcna/cyclin; PCNAR
UniProt ID: (Human) P12004, (Mouse) P17918, (Rat) P04961
Entrez Gene ID: (Dog) 477166, (Human) 5111, (Mouse) 18538, (Rat) 25737
purine-specific mismatch base pair DNA N-glycosylase activity
DNA binding
chromatin binding
damaged DNA binding
protein binding
enzyme binding
estrogen receptor binding
DNA polymerase processivity factor activity
receptor tyrosine kinase binding
dinucleotide insertion or deletion binding
MutLalpha complex binding
histone acetyltransferase binding
identical protein binding
macromolecular complex binding
DNA polymerase binding
DNA polymerase processivity factor
leading strand elongation
regulation of DNA replication
mismatch repair
chromatin organization
response to oxidative stress
heart development
translesion synthesis
epithelial cell differentiation
replication fork processing
response to estradiol
response to lipid
cellular response to UV
estrous cycle
positive regulation of DNA repair
positive regulation of DNA replication
response to cadmium ion
cellular response to hydrogen peroxide
response to dexamethasone
liver regeneration
DNA repair-dependent chromatin remodeling
response to L-glutamate
mitotic DNA replication
mitotic telomere maintenance via semi-conservative replication
negative regulation of transcription from RNA polymerase II promoter
base-excision repair, gap-filling
cellular response to xenobiotic stimulus
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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