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Invitrogen

Actin Monoclonal Antibody (ACTN05 (C4)), Biotin

2 Published Figures
195 References
View all (19) Actin antibodies

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Actin Monoclonal Antibody (ACTN05 (C4)), Biotin

Product Details

MA5-11866

Applications
Tested Dilution
Publications

Western Blot (WB)

0.5 µg/mL
View 183 publications 183 publications

Immunohistochemistry (IHC)

-
View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:400
-

Immunocytochemistry (ICC/IF)

1:10-1:200
View 3 publications 3 publications

Immunoprecipitation (IP)

2 µg
View 6 publications 6 publications
Product Specifications

Species Reactivity

Bovine, Dog, Chicken, Human, Mouse, Non-human primate, Pig, Protozoa, Rabbit, Rat

Published species

Chordate, Dog, Fish, Fruit fly, Hamster, Human, Mouse, Non-human primate, Rabbit, Rat, Xenopus, Yeast, Zebrafish

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

ACTN05 (C4)

Immunogen

Chicken gizzard actin
View immunogen

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.2 mg/mL

Amount

100 µg

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

15mM sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10985365

Product Specific Information

This antibody reacts with all six known vertebrate isoforms of actin (MW ~42 kDa), as well as cytoplasmic actin beta and actin gamma. This antibody is highly recommended for monitoring total protein load on Western blots.

Staining of formalin-fixed, paraffin-embedded tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes, followed by cooling at room temperature for 20 minutes. Suggested positive controls are HeLa cells and skeletal muscle.

Target Information

Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. In vertebrates there are three groups of actin isoforms: alpha, beta and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exists in most cell types as components of the cytoskeleton and as mediators of internal cell motility.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: a-actin; ACTA1; ACTA2; ACTB; ACTC; ACTG2; actin; actin alpha 1; actin alpha cardiac; actin alpha cardiac 1; Actin is a protein with a high degree of evolutionary conservation that undergoes polymerization to generate filaments, which subsequently assemble into interconnected networks within the cytoplasm of cells.; actin, alpha 1, skeletal muscle; actin, alpha 2, smooth muscle, aorta; Actin, alpha cardiac muscle 1; Actin, alpha skeletal muscle; actin, alpha, cardiac 1; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; Actin, cytoplasmic 1; Actin, cytoplasmic 2; Actin, cytoplasmic type 5; actin, cytoplasmic, type 5; actin, gamma 1 propeptide; actin, gamma 2, smooth muscle, enteric; actin, gamma, cytoplasmic 1; Actin, gamma-enteric smooth muscle; actin-like protein; alpha actin 1; alpha actin 2; Alpha-actin; alpha-actin cardiac; Alpha-actin-1; Alpha-actin-2; Alpha-actin-3; Alpha-cardiac actin; alpha-sma; alpha-smooth muscle actin; alphac-actin; Atcb; B-actin; Bact; actin; beta actin; beta cytoskeletal actin; Beta-actin; beta-actin FE-3; cardiac actin; cardiac muscle alpha actin 1; Cell growth-inhibiting gene 46 protein; cytoplasmic 1; cytoplasmic 2; cytoplasmic beta-actin; cytoplasmic type 5; cytoskeletal beta actin; cytoskeletal gamma-actin; epididymis luminal protein 176; gamma 1 propeptide; gamma non-muscle actin; Gamma-2-actin; Gamma-actin; gamma-enteric smooth muscle actin; GIG46; I(2)-actin; nemaline myopathy type 3; pan actin; PS1TP5-binding protein 1; RP5-1068B5.2; skeletal alpha actin; smooth muscle alpha-actin; Smooth muscle gamma-actin; type 5; unnamed protein product; vascular smooth muscle alpha-actin

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Gene Aliases: 0610041G09Rik; a-SMA; AA959943; ACT; ACT-4; ACT-5; Act4; ACTA; Acta-2; ACTA1; ACTA2; ACTA3; ACTB; ACTC; Actc-1; ACTC1; ACTE; ACTG; ACTG1; ACTG2; ACTGE; actin; Actl; ACTL3; Acts; ACTSA; ACTSG; Actsk-1; ACTVS; Actx; AL023024; alphaSMA; ASD5; ASMA; Bact; beta-actin; BKRNS; BNS; BRWS1; CFTD; CFTD1; CFTDM; CMD1R; CMH11; CMYO2A; CMYO2B; CMYO2C; CMYP2A; CMYP2B; CMYP2C; CSMH; DDS1; DFNA20; DFNA26; E430023M04Rik; E51; GAMMA-ACTIN; GIG46; HEL-176; LVNC4; MMIHS5; MPFD; NEM1; NEM2; NEM3; PS1TP5BP1; SHPM; SMalphaA; SMDYS; SMGA; THC8; VSCM; VSCM1

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UniProt ID: (Human) P68133, (Chicken) P68139, (Bovine) P68138, (Rabbit) P68135, (Pig) P68137, (Rabbit) P62740, (Chicken) P08023, (Bovine) P62739, (Human) P62736, (Rabbit) P29751, (Bovine) P60712, (Pig) Q6QAQ1, (Human) P60709, (Chicken) P60706, (Dog) O18840, (Bovine) Q3ZC07, (Chicken) P68034, (Human) P68032, (Chicken) P53478, (Human) P63261, (Bovine) P63258, (Bovine) Q5E9B5, (Chicken) P63270, (Human) P63267, (Mouse) P68134, (Rat) P68136, (Rat) P62738, (Mouse) P62737, (Rat) P60711, (Mouse) P60710, (Rat) P68035, (Mouse) P68033, (Mouse) P63260, (Rat) P63259, (Mouse) P63268, (Rat) P63269

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Entrez Gene ID: (Human) 58, (Chicken) 421534, (Bovine) 281592, (Rabbit) 100009506, (Pig) 100154254, (Dog) 488984, (Pig) 733615, (Dog) 477587, (Rabbit) 100009271, (Chicken) 423787, (Bovine) 515610, (Human) 59, (Rabbit) 100009272, (Bovine) 280979, (Pig) 414396, (Human) 60, (Chicken) 396526, (Dog) 403580, (Dog) 478250, (Bovine) 533219, (Chicken) 423298, (Human) 70, (Rabbit) 100340339, (Pig) 100152267, (Chicken) 415296, (Dog) 475923, (Human) 71, (Bovine) 404122, (Pig) 397653, (Bovine) 281595, (Chicken) 396084, (Pig) 100520667, (Human) 72, (Rabbit) 100350318, (Dog) 475792, (Mouse) 11459, (Rat) 29437, (Rat) 81633, (Mouse) 11475, (Rat) 81822, (Mouse) 11461, (Rat) 29275, (Mouse) 11464, (Mouse) 11465, (Rat) 287876, (Mouse) 11468, (Rat) 25365

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Function(s)
nucleotide binding structural constituent of cytoskeleton protein binding ATP binding hydrolase activity myosin binding ADP binding protein kinase binding kinesin binding Tat protein binding identical protein binding nitric-oxide synthase binding ATPase activity nitric-oxide synthase regulator activity nucleosomal DNA binding tau protein binding structural constituent of postsynaptic actin cytoskeleton transporter regulator activity microfilament motor activity profilin binding ubiquitin protein ligase binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding molecular_function actin and actin related protein actin or actin-binding cytoskeletal protein cytoskeletal protein
Process(es)
muscle contraction positive regulation of gene expression skeletal muscle thin filament assembly skeletal muscle fiber development mesenchyme migration regulation of blood pressure response to virus vascular smooth muscle contraction glomerular mesangial cell development positive regulation of hepatic stellate cell migration positive regulation of hepatic stellate cell contraction positive regulation of ERK1 and ERK2 cascade cellular response to transforming growth factor beta stimulus juxtaglomerular apparatus development positive regulation of hepatic stellate cell activation morphogenesis of a polarized epithelium establishment or maintenance of cell polarity regulation of transmembrane transporter activity negative regulation of protein binding adherens junction assembly apical protein localization synaptic vesicle endocytosis regulation of norepinephrine uptake protein localization to adherens junction cellular response to cytochalasin B postsynaptic actin cytoskeleton organization regulation of protein localization to plasma membrane chromatin remodeling regulation of transcription from RNA polymerase II promoter cytoskeleton organization axonogenesis positive regulation of cell proliferation substantia nigra development regulation of mitotic metaphase/anaphase transition maintenance of blood-brain barrier regulation of apoptotic process positive regulation of T cell differentiation negative regulation of cell differentiation positive regulation of cell differentiation positive regulation of myoblast differentiation positive regulation of transcription, DNA-templated cell motility positive regulation of norepinephrine uptake regulation of cell cycle regulation of G0 to G1 transition platelet aggregation regulation of transepithelial transport regulation of synaptic vesicle endocytosis positive regulation of stem cell population maintenance positive regulation of double-strand break repair via homologous recombination regulation of G1/S transition of mitotic cell cycle regulation of double-strand break repair positive regulation of double-strand break repair regulation of nucleotide-excision repair actin filament organization actin-myosin filament sliding heart contraction response to xenobiotic stimulus actin filament-based movement actomyosin structure organization negative regulation of apoptotic process response to ethanol cardiac myofibril assembly cardiac muscle tissue morphogenesis cardiac muscle contraction cytoplasmic actin-based contraction involved in cell motility angiogenesis positive regulation of cell migration sarcomere organization regulation of stress fiber assembly regulation of focal adhesion assembly cellular response to interferon-gamma positive regulation of wound healing tight junction assembly protein localization to bicellular tight junction response to mechanical stimulus response to extracellular stimulus response to lithium ion cell growth skeletal muscle fiber adaptation response to steroid hormone retina homeostasis ATP-dependent chromatin remodeling retina development in camera-type eye cellular response to electrical stimulus metabolic process response to drug actin-mediated cell contraction response to calcium ion biological_process
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