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Invitrogen
CD40 Monoclonal Antibody (LOB7/6), FITC
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-16775
Product Specifications
Species Reactivity
Dog,
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
LOB7/6
Immunogen
CXD40Ig(Fc) fusion protein containing the EC region of human CD40 and Fc region of human IgG
Conjugate
FITC
FITC
FITC
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
0.1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS with 1% BSA
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Target Information
CD40 is a single-chain glycoprotein and a member of the tumor necrosis factor receptor (TNFR) family, exhibiting significant homology to the Hodgkin's disease-associated antigen, CD30. It is expressed by B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells, as well as some epithelial cells, carcinomas, and lymphoid dendritic cells. Notably, CD40 is present on all B cells except plasma cells. CD40 plays a crucial role in regulating B cell development and maturation, inducing immunoglobulin isotype-switching, and protecting B cells from surface Ig-induced apoptosis when combined with other signals such as IL-4. It promotes proliferation and is essential for T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. The interaction between CD40 and its ligand CD154 (gp39) on T cells is vital for T-B cell crosstalk, costimulation, and immune regulation. Adaptor protein TNFR2 interacts with CD40, mediating signal transduction, while the AT-hook transcription factor AKNA is reported to regulate CD40 expression, which may be important for homotypic cell interactions. Additionally, the interaction between CD40 and its ligand is necessary for amyloid-beta-induced microglial activation, suggesting a role in the early pathogenesis of Alzheimer's disease. Two alternatively spliced transcript variants encoding distinct isoforms of CD40 have been identified. Diseases associated with CD40 dysfunction include Type 3 Hyper-IgM immunodeficiency and CD40 ligand deficiency, highlighting its importance in immune and inflammatory responses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B cell surface antigen CD40; B cell-associated molecule; B-cell surface antigen CD40; CD antigen CD40; CD40 antigen (TNF receptor superfamily member 5); CD40 molecule, TNF receptor superfamily member 5; CD40L receptor; Immunoglobulin M; ImmunoglobulinM; MGC9013; receptor for ligand CD154; sCD40; soluble CD40; tumor necrosis factor receptor superfamily, member 5
Gene Aliases: Bp50; CDW40; p50; TNFRSF5
UniProt ID: (Dog) Q7YRL5, (Human) P25942
Entrez Gene ID: (Dog) 403469, (Human) 958
B cell activation
regulation of immune response
immune response-regulating cell surface receptor signaling pathway
cellular calcium ion homeostasis
defense response
inflammatory response
JAK-STAT cascade
regulation of gene expression
B cell mediated immunity
CD40 signaling pathway
platelet activation
B cell proliferation
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of MAPK cascade
positive regulation of blood vessel endothelial cell migration
positive regulation of angiogenesis
positive regulation of transcription from RNA polymerase II promoter
positive regulation of developmental process
positive regulation of multicellular organismal process
protein-containing complex assembly
cellular response to mechanical stimulus
positive regulation of protein kinase C signaling
positive regulation of interleukin-4-mediated signaling pathway
positive regulation of endothelial cell apoptotic process
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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