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Invitrogen
CD43 Monoclonal Antibody (W3/13)
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Product Details
MA5-16978
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
W3/13
Immunogen
Rat thymocyte membrane glycoproteins
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
250 µg
Purification
Protein G
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Heat-mediated antigen retrieval is recommended for the staining of paraffin sections. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Target Information
CD43, also known as leukosialin or sialophorin, is a transmembrane mucin-like protein characterized by a high negative charge. It is expressed on the surface of most hematopoietic cells, excluding resting B lymphocytes. CD43 serves both adhesive and anti-adhesive functions, contributing to a repulsive barrier that can interfere with cellular adhesion while also promoting leukocyte aggregation in certain contexts. CD43 is involved in the activation of T cells, B cells, NK cells, and monocytes. Its expression decreases upon activation due to proteolytic processing. The counter-receptor for CD43 is CD169/SIGLEC-1, which is expressed on macrophages. CD43 interacts with actin-binding proteins ezrin and moesin, playing a regulatory role in remodeling T-cell morphology and regulating cell-cell interactions during lymphocyte traffic. It enhances LFA-1 adhesiveness while counteracting LFA-1 induction via other receptors. CD43 signaling induces the functionally active tumor suppressor p53 protein, but in cases of p53 and ARF deficiency, it can promote tumor proliferation and viability. CD43 is an important modulator of leukocyte functions, with dysfunctions associated with diseases such as Wiscott-Aldrich Syndrome and Adenoid Basal Cell Carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: leukocyte sialoglycoprotein; leukosianin; sialophorin gpL115; W3/13 antigen
Gene Aliases: Cd43; gpL115; Lsn; Lsn1
Entrez Gene ID: (Rat) 24796
response to protozoan
negative regulation of type IV hypersensitivity
positive regulation of protein phosphorylation
T-helper 1 cell lineage commitment
chronic inflammatory response
negative regulation of cell adhesion
cell surface receptor signaling pathway
regulation of gene expression
T cell costimulation
positive regulation of tumor necrosis factor production
positive regulation of T cell proliferation
monocyte activation
negative regulation of T cell proliferation
defense response to bacterium
negative thymic T cell selection
regulation of defense response to virus
regulation of immune response
T cell receptor signaling pathway
regulation of T cell activation
negative regulation of T cell activation
leukocyte tethering or rolling
thymocyte aggregation
apoptotic signaling pathway
regulation of T cell migration
positive regulation of T cell migration
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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