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Invitrogen
LAMP1 Monoclonal Antibody (H4A3), Alexa Fluor™ 488
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FIGURE: 1 / 4
LAMP1 Antibody (MA5-18121) in ICC/IF
Knockdown of LAMP1 was achieved by transfecting HeLa cells with LAMP1 specific siRNAs (Silencer® select Product # s8080, s8081 ). Immunofluorescence analysis was performed using untransfected HeLa cells (panels a, d), transfected with non-specific scrambled siRNA (panels b,e) and transfected with LAMP1 specific siRNAs (panel c,f). Cells were fixed, permeabilized, and probed with LAMP1 Monoclonal Antibody (H4A3), Alexa Fluor 488 (Product # MA5-18121, 1:250 dilution). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with D... View More
Product Details
MA5-18121
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
H4A3
Immunogen
Human PBMC
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
0.13 mg/mL
Purification
Size-exclusion chromatography
Storage buffer
PBS with 0.2% BSA
Contains
15mM sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody recognizes an extracellular/luminal epitope of CD107a, an approximately 100-120 kDa glycoprotein expressed mainly on lysosomal, but also on the plasma membrane.
Target Information
LAMP1 (CD107a, lysosome-associated membrane protein-1) together with LAMP-2, is a major constituent of lysosomal membrane, 1-2% of total CD107a is found also on the plasma membrane. LAMP1 is a heavily glycosylated membrane protein which contains a putative signal peptide, 18 sites for N-linked glycosylation, a single membrane-spanning segment and a short (11 amino acid) cytosolic tail. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes. LAMP1 is a type 1 integral membrane protein that is transported from trans-Golgi network to endosomes and then lysosomes. Upon cell activation, LAMP1 transfer to the plasma membrane is dependent on a carboyxl-terminal tyrosine based motif (YXXI). Perturbation in the spacing between the tyrosine based motif relative to the membrane abolishes lysosome localization of LAMP1, and this mutant protein then cycles between the plasma membrane and the endosome. Cell surface LAMP1 (and LAMP2) have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore, they are possibly involved in the adhesion of PBMC to the site of inflammation. Increased LAMP1 immunoreactivity is observed in neurons and glial cells surrounding senile plaques in Alzheimer's Disease (AD) cases, and is localized in medullary epithelial cells, single macrophages and lymphocytes in acute thymic involution. LAMP1 is a good marker of mast cell activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 120 kDa lysosomal membrane glycoprotein; CD107 antigen-like family member A; CD107a; Lamp I; LAMP-1; LGP-120; LGP-A; lysosomal membrane glycoprotein 1; Lysosomal membrane glycoprotein A; Lysosome-associated membrane glycoprotein 1; LYSOSOME-ASSOCIATED MEMBRANE GLYCOPROTEIN 1 PRECURSOR (LAMP-1) (LGP-A) (LGP-120) (CD107A) (P2B); lysosome-associated membrane protein 1; membrane glycoprotein; P2B; unnamed protein product
Gene Aliases: AI196048; CD107a; Lamp-1; LAMP1; LAMPA; LGP120
UniProt ID: (Human) P11279, (Mouse) P11438
Entrez Gene ID: (Human) 3916, (Mouse) 16783
lysosomal lumen acidification
lysosomal lumen pH elevation
positive regulation of natural killer cell degranulation
positive regulation of natural killer cell mediated cytotoxicity
viral entry into host cell
protein stabilization
establishment of protein localization to organelle
Golgi to lysosome transport
granzyme-mediated programmed cell death signaling pathway
regulation of organelle transport along microtubule
hydrogen ion transmembrane transport
spermatogenesis
granzyme-mediated apoptotic signaling pathway
autophagic cell death
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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