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Invitrogen
VGLUT2 Monoclonal Antibody (N29/29)
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FIGURE: 1 / 3
VGLUT2 Antibody (MA5-27613) in ICC/IF
Immunofluorescent analysis of VGLUT2 in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with VGLUT2 monoclonal antibody (Product # MA5-27613) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:200, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) VGLUT2 Antibody, D) Merged image. Magnificat... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-27613
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N29/29
Immunogen
Fusion protein amino acids 501-582 (cytoplasmic C-terminus) of rat VGlut2
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-27613 was sufficient for detection of VGLut2 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 60kDa.
This antibody was formerly sold as clone S29-29
Target Information
VGLU2 mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. This protein may also mediate the transport of inorganic phosphate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: differentation-associated Na-dependent inorganic phosphate cotransporter; differentiation-associated BNPI; differentiation-associated Na(+)-dependent inorganic phosphate cotransporter; differentiation-associated Na-dependent inorganic phosphate cotransporter; DNPI; solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6; solute carrier family 17 (vesicular glutamate transporter), member 6; solute carrier family 17 member 6; unnamed protein product; VGlU2; VGLUT-2
Gene Aliases: 2900073D12Rik; DNPI; VGLUT2
UniProt ID: (Human) Q9P2U8, (Mouse) Q8BLE7, (Rat) Q9JI12
Entrez Gene ID: (Human) 57084, (Mouse) 140919, (Rat) 84487
chloride channel activity
L-glutamate transmembrane transporter activity
neurotransmitter transporter activity
sodium:phosphate symporter activity
secondary active transmembrane transporter activity
potassium:proton antiporter activity
transmembrane transporter activity
L-glutamate uniporter activity
phosphate ion uniporter activity
phosphate ion transport
neurotransmitter transport
L-glutamate transport
synaptic transmission, glutamatergic
sodium ion transmembrane transport
sodium-dependent phosphate transport
regulation of synapse structure or activity
L-glutamate import
phosphate ion homeostasis
transmembrane transport
potassium ion transmembrane transport
neurotransmitter loading into synaptic vesicle
chloride transmembrane transport
hydrogen ion transmembrane transport
hyaloid vascular plexus regression
neurotransmitter uptake
neural retina development
hippocampus development
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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