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Invitrogen
HSP40 Monoclonal Antibody (3B9.E6)
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FIGURE: 1 / 5
HSP40 Antibody (MA5-27685) in ICC/IF
Immunofluorescent analysis of HSP40 in human HaCaT cells. Sample was fixed with 100% cold methanol (10 minutes at -20°C), incubated with HSP40 monoclonal antibody (Product # MA5-27685) using a dilution of 1:100 (1 hour at RT), and followed by FITC Goat Anti-Mouse (green) secondary antibody at a dilution of 1:50 (1 hour at RT).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-27685
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
3B9.E6
Immunogen
Recombinant Protein human HSP40 (Hdj1)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunohistochemistry samples may be prepared using cold 100% methanol at -20C for fixation.
0.5 µg/mL of MA5-27685 was sufficient for detection of HSP40 (HDJ1) in 15 µg of HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 40kDa. Does not cross-react with HDJ2 or YDJ1.
Target Information
TCF-9 (transcription factor 9), also known as C2orf3 (chromosome 2 open reading frame 3), GCF (GC-rich sequence DNA-binding factor) or DNABF, is a 781 amino acid nuclear protein that belongs to the GCF family. Widely expressed, GCF binds the GC-rich sequences of beta-Actin, EGFR and calcium-dependent protease (CANP) promoters. GCF contains multiple phosphoserine and phosphothreonine residues, and two GCF isoforms are produced due to alternative splicing events. GCF is considered a candidate for susceptibility to dyslexia (DYX3) as both genes reside in close proximity on human chromosome 2. Chromosome 2 is the second largest human chromosome and consists of 237 million bases, encodes over 1,400 genes and makes up approximately 8% of the human genome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DnaJ (Hsp40) homolog, subfamily B, member 1; dnaJ protein homolog 1; DNAJB1; heat shock 40 kDa protein 1; Heat shock protein; HSP; HSP 40; human DnaJ protein 1; NDAJB1; radial spoke 16 homolog B; unnamed protein product
Gene Aliases: 0610007I11Rik; DjB1; Hdj1; Hsp40; HSPF1; RSPH16B; Sis1
UniProt ID: (Human) P25685, (Mouse) Q9QYJ3
Entrez Gene ID: (Human) 3337, (Mouse) 81489, (Rat) 361384
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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