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Invitrogen
HSP40 Monoclonal Antibody (3B9.E6), PerCP
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FIGURE: 1 / 2
HSP40 Antibody (MA5-45178) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using HaCaT cells. Fixation involved Cold 100% methanol for 10 minutes at -20°C. Samples were incubated with Hsp40 monoclonal antibody (Product # MA5-45178) at 1:100 for 1 hour at RT, followed by FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.
Product Details
MA5-45178
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
3B9.E6
Immunogen
Recombinant Protein HSP40 (Hdj1)
Conjugate
Excitation/Emission Max
482/675 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
0.5 µg/mL of MA5-45178 was sufficient for detection of HSP40 (HDJ1) in 15 µg of HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 40kDa. Does not cross-react with HDJ2 or YDJ1.
Target Information
TCF-9 (transcription factor 9), also known as C2orf3 (chromosome 2 open reading frame 3), GCF (GC-rich sequence DNA-binding factor) or DNABF, is a 781 amino acid nuclear protein that belongs to the GCF family. Widely expressed, GCF binds the GC-rich sequences of beta-Actin, EGFR and calcium-dependent protease (CANP) promoters. GCF contains multiple phosphoserine and phosphothreonine residues, and two GCF isoforms are produced due to alternative splicing events. GCF is considered a candidate for susceptibility to dyslexia (DYX3) as both genes reside in close proximity on human chromosome 2. Chromosome 2 is the second largest human chromosome and consists of 237 million bases, encodes over 1,400 genes and makes up approximately 8% of the human genome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DnaJ (Hsp40) homolog, subfamily B, member 1; DnaJ homolog subfamily B member 1; DnaJ protein homolog 1; DNAJB1; hDj-1; Heat shock 40 kDa protein 1; Heat shock protein; HSP; HSP 40; HSP40; Human DnaJ protein 1; NDAJB1; radial spoke 16 homolog B; unnamed protein product
Gene Aliases: 0610007I11Rik; DjB1; DNAJ1; DNAJB1; HDJ1; Hsp40; HSPF1; RSPH16B; Sis1
UniProt ID: (Human) P25685, (Mouse) Q9QYJ3
Entrez Gene ID: (Human) 3337, (Rat) 361384, (Mouse) 81489
negative regulation of transcription from RNA polymerase II promoter
protein folding
response to unfolded protein
forebrain development
cellular response to heat
negative regulation of inclusion body assembly
regulation of cellular response to heat
positive regulation of protein folding
positive regulation of ATPase activity
chaperone mediated protein folding requiring cofactor
chaperone cofactor-dependent protein refolding
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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