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Invitrogen
GluR1 Monoclonal Antibody (N355/1)
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FIGURE: 1 / 5
GluR1 Antibody (MA5-27694) in ICC/IF
Immunofluorescent analysis of GluR1 in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with GluR1 monoclonal antibody (Product # MA5-27694) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) GluA1/GluR1 Antibody, D) Merged image. Magnifi... View More
Product Details
MA5-27694
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N355/1
Immunogen
Fusion protein amino acids 1-389 (extracellular N-terminus) of rat GluA1/GluR1
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-27694 was sufficient for detection of GluA1/GluR1 in 20 µg of mouse brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 100kDa. Does not cross-react with GluR2.
This antibody was formerly sold as clone S355-1
Target Information
GluR1 (glutamate receptor 1) is an ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter, L-glutamate, induces a conformation change that leads to the opening of the cation channel and converts the chemical signal to an electrical impulse. Then, the receptor desensitizes rapidly and enters a transiet inactive state characterized by the presence of a bound agonist. In the presence of CACNG4, CACNG7, or CACNG8 GluR1 shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: AI853806; AMPA 1; AMPA-selective glutamate receptor 1; GluA1; GLUH 1; GluR K1; GluR-1; GluR-A; GluR-K1; GluR1; GluRK1; glutamate receptor; Glutamate receptor 1; glutamate receptor A; Glutamate receptor ionotropic, AMPA 1; glutamate receptor subunit GluR1; glutamate receptor, ionotropic, AMPA 1; glutamate receptor, ionotropic, AMPA1 (alpha 1); GPRC1A; Gria 1; GRM1A; HGBR1; HIPA; MGC13325; MGC133252; NMDA1; NMDAR1; NR1; OTTHUMP00000160643; OTTHUMP00000165781; OTTHUMP00000224241; OTTHUMP00000224242; OTTHUMP00000224243; RP11-350O14.1
Gene Aliases: 2900051M01Rik; Glr-1; Glr1; GLUA1; GLUH1; Glur-1; GluR-A; gluR-K1; GLUR1; GLURA; GRIA1; HBGR1; HIPA1
UniProt ID: (Human) P42261, (Rat) P19490, (Mouse) P23818
Entrez Gene ID: (Human) 2890, (Rat) 50592, (Mouse) 14799
AMPA glutamate receptor activity
excitatory extracellular ligand-gated ion channel activity
extracellular-glutamate-gated ion channel activity
protein binding
glutamate receptor activity
PDZ domain binding
G-protein alpha-subunit binding
ionotropic glutamate receptor activity
adenylate cyclase binding
protein kinase binding
protein domain specific binding
small GTPase binding
myosin V binding
G-protein beta-subunit binding
beta-2 adrenergic receptor binding
identical protein binding
protein homodimerization activity
protein kinase A binding
receptor activity
ion channel activity
transmembrane signal receptor
ER to Golgi vesicle-mediated transport
signal transduction
chemical synaptic transmission
long-term memory
receptor internalization
ion transmembrane transport
ionotropic glutamate receptor signaling pathway
COPII vesicle coating
long term synaptic depression
regulation of receptor recycling
response to toxic substance
response to lithium ion
response to organic cyclic compound
neuronal action potential
spinal cord development
response to estradiol
synaptic transmission, glutamatergic
response to cocaine
response to drug
response to peptide hormone
positive regulation of membrane potential
response to arsenic-containing substance
regulation of synaptic plasticity
modulation of synaptic transmission
positive regulation of synaptic transmission
response to electrical stimulus
response to fungicide
cellular response to amino acid stimulus
cellular response to dsRNA
cellular response to growth factor stimulus
cellular response to peptide hormone stimulus
cellular response to organic cyclic compound
cellular response to amine stimulus
transport
ion transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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