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Invitrogen
CaV1.2 Monoclonal Antibody (S57)
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FIGURE: 1 / 7
CaV1.2 Antibody (MA5-27717) in ICC/IF
Immunofluorescent analysis of Cav1.2 in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with Cav1.2 monoclonal antibody (Product # MA5-27717) using a dilution of 1:200 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:200 (1 hour at RT). Images are shown as follows: (A) Phalloidin Texas Red F-Actin stain, DAPI (blue) nuclear stain, (B) Anti-Cav1,2 Antibody, (C) Merged image (A) DAPI (blue) nuclear stain, (B) Phall... View More
Product Details
MA5-27717
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Hamster,
Human,
Mouse,
Rabbit,
Rat
Published species
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
S57
Immunogen
Fusion protein amino acids 1507-1733 (intracellular carboxyl terminus) of rabbit Cav1.2
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-27717 was sufficient for detection of Cav1.2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 240kDa (varies with cell background due to glycosylation).
Clone formerly known as S57-46.
Target Information
CACNA1C encodes an alpha-1 subunit of a voltage-dependent calcium channel. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization. The alpha-1 subunit consists of 24 transmembrane segments and forms the pore through which ions pass into the cell. The calcium channel consists of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. There are multiple isoforms of each of these proteins, either encoded by different genes or the result of alternative splicing of transcripts. The protein encoded by CACNA1C binds to and is inhibited by dihydropyridine. Alternative splicing results in many transcript variants encoding different proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: brain class C; CaCB receptor; CACB-receptor; CACH3; CACN4; CACNA 1D; CACNA1C intronic transcript 2; CACNA1C intronic transcript 2 (non-protein coding); CACNL1A2; calcium channel voltage-dependent alpha1c subunit; calcium channel, cardic dihydropyridine-sensitive, alpha-1 subunit; Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle; calcium channel, voltage-dependent, alpha 1C subunit; calcium channel, voltage-dependent, L type, alpha 1C subunit; CaV1.2; DHP receptor; DHPR, alpha-1 subunit; L-type calcium channel alpha-1 subunit; long-NT; LTCC; MBC; MELC-CC; MGC120730; Mouse brain class C; neuronal voltage-gated calcium channel alpha 1C subunit; Rat brain class C; RBC; skeletal muscle-specific calcium channel; smooth muscle calcium channel blocker; Smooth muscle calcium channel blocker receptor; unnamed protein product; voltage-dependent L-type Ca2+ channel alpha 1 subunit; Voltage-dependent L-type calcium channel subunit alpha-1C; Voltage-gated calcium channel subunit alpha Cav1.2
Gene Aliases: CACB; CACH2; CACN2; CACNA1C; CACNA1C-IT2; CACNL1A1; CaV1.2; CCHL1A1; D930026N18Rik; I79_006562; LQT8; MBC; MELC-CC; NEDHLSS; RATIVS302; TS; TS. LQT8
UniProt ID: (Human) Q13936, (Rabbit) P15381, (Mouse) Q01815, (Rat) P22002
Entrez Gene ID: (Rabbit) 100144322, (Human) 775, (Rabbit) 100101555, (Mouse) 12288, (Chinese hamster) 100754788, (Rat) 24239
ion channel activity
voltage-gated calcium channel activity
calcium channel activity
protein binding
calmodulin binding
high voltage-gated calcium channel activity
metal ion binding
alpha-actinin binding
voltage-gated calcium channel activity involved in cardiac muscle cell action potential
voltage-gated calcium channel activity involved in AV node cell action potential
voltage-gated ion channel activity
enzyme binding
protein domain specific binding
ion channel binding
protein phosphatase 2A binding
voltage-gated ion channel
immune system development
ion transport
calcium ion transport
muscle contraction
positive regulation of cytosolic calcium ion concentration
heart development
regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
ion transmembrane transport
embryonic forelimb morphogenesis
camera-type eye development
positive regulation of adenylate cyclase activity
positive regulation of muscle contraction
transmembrane transport
calcium ion transport into cytosol
cardiac conduction
calcium ion transmembrane transport via high voltage-gated calcium channel
calcium ion transmembrane transport
cardiac muscle cell action potential involved in contraction
membrane depolarization during cardiac muscle cell action potential
membrane depolarization during AV node cell action potential
cell communication by electrical coupling involved in cardiac conduction
regulation of heart rate by cardiac conduction
calcium ion import across plasma membrane
regulation of ventricular cardiac muscle cell action potential
membrane depolarization during atrial cardiac muscle cell action potential
transport
cellular calcium ion homeostasis
smooth muscle contraction
chemical synaptic transmission
adult walking behavior
regulation of blood pressure
visual learning
calcium ion regulated exocytosis
regulation of vasoconstriction
insulin secretion
growth hormone secretion
regulation of ion transmembrane transport
glucose homeostasis
regulation of organ growth
smooth muscle contraction involved in micturition
calcium ion import
membrane depolarization during action potential
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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