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Invitrogen
HCN2 Monoclonal Antibody (S71)
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FIGURE: 1 / 3
HCN2 Antibody (MA5-27718) in IHC (P)
Immunohistochemistry analysis of HCN2 in paraffin-embedded human hippocampus. Sample was fixed with Bouin's Fixative Solution, incubated with HCN2 monoclonal antibody (Product # MA5-27718) using a dilution of 1:100 (1 hour at RT), and followed by FITC Goat Anti-Mouse (green) (1 hour at RT) at a dilution of 1:50.
Product Details
MA5-27718
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
S71
Immunogen
Fusion protein amino acids 761-863 of rat HCN2
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-27718 was sufficient for detection of HCN2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 95kDa. No cross-reactivity against HCN1.
Target Information
Hyperpolarization-activated cation channels of the HCN gene family such as HCN2, contribute to spontaneous rhythmic activity in both heart and brain. HCN2 is a member of a family of pacemaker channels activated by hyperpolarization and regulated by cyclic nucleotides. HCN1 and HCN2 play an important role for motor learning and neuronal integration by cerebellar Purkinje cells; as well as, shaping autonomous activity of single neurons and the periodicity of network oscillations. The HCN1 expression is highly enriched in cerebral cortex, hippocampus, cerebellum, and facial motor nucleus. HCN2 is highly abundant in mamillary bodies, pontine nucleus, ventral cochlear nucleus, and nucleus of the trapezoid body. These variations in regional specificity of HCN channels could generate important differences in neuronal pacemaker activity across brain systems.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: BCNG-2; Brain cyclic nucleotide-gated channel 2; HAC-1; Hyperpolarization-activated cation channel 1; Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2
Gene Aliases: BCNG2; HAC1; Hcn2; trls
UniProt ID: (Mouse) O88703, (Rat) Q9JKA9
Entrez Gene ID: (Mouse) 15166, (Rat) 114244
nucleotide binding
ion channel activity
intracellular cAMP activated cation channel activity
voltage-gated ion channel activity
voltage-gated sodium channel activity
voltage-gated potassium channel activity
potassium channel activity
sodium channel activity
protein binding
PDZ domain binding
cAMP binding
protein complex binding
protein complex scaffold
identical protein binding
ion channel
transporter
transport
ion transport
potassium ion transport
sodium ion transport
regulation of ion transmembrane transport
sodium ion transmembrane transport
regulation of membrane potential
positive regulation of heart contraction
transmembrane transport
cellular response to cAMP
cellular response to cGMP
potassium ion transmembrane transport
inflammatory response
brain development
aging
response to hormone
response to drug
cellular response to steroid hormone stimulus
response to cisplatin
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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