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Invitrogen
HCN2 Monoclonal Antibody (S71), PE
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FIGURE: 1 / 6
HCN2 Antibody (MA5-45406) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% PFA for 15 min. Samples were incubated with HCN2 monoclonal antibody (Product # MA5-45406) at 1:50 for overnight at 4°C with slow rocking, followed by AlexaFluor 488 at 1:1,000 for 1 hour at RT. Counterstain used was Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) HCN2 antibody (D) Composite.
Product Details
MA5-45406
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
S71
Immunogen
Fusion protein amino acids 761-863 (cytoplasmic C-terminus) of rat HCN2
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-45406 was sufficient for detection of HCN2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 95kDa. No cross-reactivity against HCN1.
Target Information
Hyperpolarization-activated cation channels of the HCN gene family such as HCN2, contribute to spontaneous rhythmic activity in both heart and brain. HCN2 is a member of a family of pacemaker channels activated by hyperpolarization and regulated by cyclic nucleotides. HCN1 and HCN2 play an important role for motor learning and neuronal integration by cerebellar Purkinje cells; as well as, shaping autonomous activity of single neurons and the periodicity of network oscillations. The HCN1 expression is highly enriched in cerebral cortex, hippocampus, cerebellum, and facial motor nucleus. HCN2 is highly abundant in mamillary bodies, pontine nucleus, ventral cochlear nucleus, and nucleus of the trapezoid body. These variations in regional specificity of HCN channels could generate important differences in neuronal pacemaker activity across brain systems.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BCNG-2; Brain cyclic nucleotide-gated channel 2; HAC-1; hyperpolarization activated cyclic nucleotide gated potassium channel 2; Hyperpolarization-activated cation channel 1; Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2
Gene Aliases: BCNG-2; BCNG2; EIG17; FEB2; GEFSP11; HAC-1; HAC1; HCN2; trls
UniProt ID: (Human) Q9UL51, (Mouse) O88703, (Rat) Q9JKA9
Entrez Gene ID: (Human) 610, (Mouse) 15166, (Rat) 114244
nucleotide binding
ion channel activity
intracellular cAMP activated cation channel activity
voltage-gated sodium channel activity
voltage-gated potassium channel activity
potassium channel activity
sodium channel activity
protein binding
PDZ domain binding
cAMP binding
identical protein binding
macromolecular complex binding
binding, bridging
voltage-gated ion channel activity
protein complex binding
protein complex scaffold
ion channel
transporter
regulation of membrane depolarization
ion transport
potassium ion transport
sodium ion transport
cell-cell signaling
response to xenobiotic stimulus
response to hormone
ion transmembrane transport
sodium ion transmembrane transport
regulation of membrane potential
transmembrane transport
cellular response to cAMP
cellular response to cGMP
potassium ion transmembrane transport
ammonium transmembrane transport
membrane depolarization during cardiac muscle cell action potential
sodium ion import across plasma membrane
cellular response to aldosterone
potassium ion import across plasma membrane
transport
regulation of ion transmembrane transport
positive regulation of heart contraction
inflammatory response
brain development
aging
response to drug
cellular response to steroid hormone stimulus
response to cisplatin
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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