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Invitrogen
RLTPR Monoclonal Antibody (EM-53), PE
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FIGURE: 1 / 1
RLTPR Antibody (MA5-28631) in Flow
Separation of RLTPR transfected cells stained using anti-RLTPR (EM-53) PE Monoclonal antibody (Product # MA5-28631), concentration in sample 27 µg/mL (red) from unstained RLTPR transfected cells (black) in flow cytometry analysis (intracellular staining).
Product Details
MA5-28631
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
EM-53
Immunogen
Murine Rltpr (amino acids 1147-1397)
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.1 mg/mL
Amount
100 µg
Purification
Size-exclusion chromatography
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
15mM sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody recognizes RLTPR / CARMIL2, an intracellular protein playing a role in actin filament elongation.
Target Information
RLTPR/CARMIL2 (RGD motif, leucine rich repeats, tropomodulin domain and proline-rich containing; capping protein regulator and myosin 1 linker 2), also known as LRRC16C, is a cytosolic protein, which with high affinity binds CAPZA2 (capping protein muscle actin Z-line alpha 2) and decreases CAPZA2 affinity for actin barbed ends. RLTPR/CARMIL2 increases the rate of actin filament elongation from seeds in the presence of CAPZA2, however, seems unable to nucleate filaments. Its interaction with CAPZA2 is essential for lamellipodial protrusion and cell translocation. RLTPR/CARMIL2 is crucial for T cell costimulation via CD28 and this property seems to be independent on its actin-uncapping function. The lack of functional RLTPR/CARMIL2 molecules impeded the differentiation toward Th1 and Th17 fates of both human and murine CD4+ T cells and leads to combined immunodeficiency. Expression of RLTPR/CARMIL2 was also detected in human and murine B cells, but it seems not to be involved in BCR-mediated signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Capping protein regulator and myosin 1 linker 2; Capping protein, Arp2/3 and myosin-I linker protein 2; cytoskeleton protein; F-actin-uncapping protein RLTPR; leucine rich repeat containing 16C; Leucine-rich repeat-containing protein 16C; RGD motif, leucine rich repeats, tropomodulin domain and proline-rich containing; RGD, leucine-rich repeat, tropomodulin and proline-rich containing protein; RGD, leucine-rich repeat, tropomodulin and proline-rich-containing protein
Gene Aliases: CARMIL2; CARMIL2b; CG1399-PB; D130029J02Rik; Gm585; IMD58; LRRC16C; RLTPR
UniProt ID: (Human) Q6F5E8
Entrez Gene ID: (Human) 146206, (Mouse) 234695
establishment or maintenance of cell polarity
positive regulation of lamellipodium assembly
cell migration
positive regulation of cell migration
regulation of Arp2/3 complex-mediated actin nucleation
wound healing, spreading of cells
actin filament network formation
establishment or maintenance of monopolar cell polarity
positive regulation of extracellular matrix disassembly
positive regulation of ruffle assembly
positive regulation of lamellipodium organization
negative regulation of barbed-end actin filament capping
maintenance of cell polarity
positive regulation of interferon-gamma production
positive regulation of T cell proliferation
establishment of protein localization
positive regulation of regulatory T cell differentiation
thymus development
homeostasis of number of cells
T cell receptor signaling pathway
positive regulation of interleukin-2 secretion
positive regulation of interferon-gamma secretion
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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