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Invitrogen
CD28 Recombinant Rabbit Monoclonal Antibody (007)
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FIGURE: 1 / 1
CD28 Antibody (MA5-29103) in Flow
Flow cytometric analysis of Human CD28 expression on human whole blood lymphocytes. Cells were stained with CD28 Recombinant Rabbit Monoclonal Antibody (7) (Product # MA5-29103), then a FITC-conjugated Secondary antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.
Product Details
MA5-29103
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
007
Immunogen
Recombinant Human CD28/TP44 protein (1-152).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Human CD28/TP44.
Target Information
CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing T cells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD28; MGC138290; sCD28; soluble CD28; T-cell-specific homodimer surface protein precursor; T-cell-specific surface glycoprotein CD28; T44; TP44; unnamed protein product
Gene Aliases: CD28; IMD123; Tp44
UniProt ID: (Human) P10747
Entrez Gene ID: (Human) 940
positive regulation of cytokine production
immune system process
positive regulation of inflammatory response to antigenic stimulus
transcription from RNA polymerase II promoter
immune response
humoral immune response
cell surface receptor signaling pathway
positive regulation of gene expression
negative regulation of gene expression
T cell costimulation
positive regulation of interleukin-10 production
positive regulation of interleukin-2 production
positive regulation of interleukin-4 production
CD4-positive, alpha-beta T cell proliferation
T cell proliferation
positive regulation of T cell proliferation
T cell activation
regulation of T cell proliferation
negative regulation of apoptotic process
protein kinase B signaling
negative thymic T cell selection
regulatory T cell differentiation
positive regulation of viral genome replication
regulation of regulatory T cell differentiation
positive regulation of translation
positive regulation of mitotic nuclear division
positive regulation of transcription from RNA polymerase II promoter
positive regulation of isotype switching to IgG isotypes
T cell receptor signaling pathway
positive regulation of T cell activation
positive regulation of protein kinase B signaling
apoptotic signaling pathway
positive regulation of CD4-positive, alpha-beta T cell proliferation
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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