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Invitrogen
CD58 Recombinant Rabbit Monoclonal Antibody (083)
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FIGURE: 1 / 1
CD58 Antibody (MA5-29119) in Flow
Flow cytometric analysis of Human CD58 expression on human whole blood lymphocytes. Cells were stained with CD58 Recombinant Rabbit Monoclonal Antibody (83) (Product # MA5-29119), then a FITC-conjugated Secondary antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.
Product Details
MA5-29119
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
083
Immunogen
Recombinant Human LFA-3/CD58 Protein (Met1-Arg215)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Human LFA-3/CD58.
Target Information
CD58 is a member of the immunoglobulin superfamily and exists both as a transmembrane protein and a GPI-linked extracellular protein, with cells capable of expressing both forms concurrently. Additionally, soluble CD58 has been detected in serum, urine, and synovial fluid, particularly in patients with rheumatoid arthritis. CD58 is widely expressed on non-hematopoietic and most hematopoietic cells. CD58 serves as a ligand for the CD2 protein on T lymphocytes, playing a crucial role in adhesion and activation of T cells. The interaction between CD58 and CD2 initiates a signaling pathway that includes phosphorylation and leads to cytokine release. This interaction is important for T cell activation, cytokine production, cytotoxicity, and adhesion to various cell types. The protein is localized to the plasma membrane, and alternatively spliced transcript variants have been described, indicating diverse functional roles. CD58's involvement in immune responses highlights its significance in both normal physiological processes and disease states.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Ag3; CD305; CD58; CD58 antigen, (lymphocyte function-associated antigen 3); FLJ23181; FLJ43722; LAIR-1; LAIR1; Lymphocyte function-associated antigen 3; lymphocyte function-associated antigen 3 isoform delta D2; RP5-1086K13.2; Surface glycoprotein LFA-3; unnamed protein product
Gene Aliases: ag3; CD58; LFA-3; LFA3
UniProt ID: (Human) P19256
Entrez Gene ID: (Human) 965
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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