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Invitrogen
CD58 Recombinant Rabbit Monoclonal Antibody (083), FITC
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FIGURE: 1 / 4
CD58 Antibody (MA5-46751) in Flow
Flow cytometry analysis of CD58 in human whole blood lymphocytes using CD58, FITC Recombinant Monoclonal (Product # MA5-46751). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.
Product Details
MA5-46751
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
083
Immunogen
Recombinant protein ecoding Human CD58 (Met1-Arg215).
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
100 µg/mL
Purification
Protein A
Storage buffer
PBS with 0.5% BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human CD58 (Met1-Arg215) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
Storage: This antibody can be stored at 2C-8C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Target Information
CD58 is a member of the immunoglobulin superfamily and exists both as a transmembrane protein and a GPI-linked extracellular protein, with cells capable of expressing both forms concurrently. Additionally, soluble CD58 has been detected in serum, urine, and synovial fluid, particularly in patients with rheumatoid arthritis. CD58 is widely expressed on non-hematopoietic and most hematopoietic cells. CD58 serves as a ligand for the CD2 protein on T lymphocytes, playing a crucial role in adhesion and activation of T cells. The interaction between CD58 and CD2 initiates a signaling pathway that includes phosphorylation and leads to cytokine release. This interaction is important for T cell activation, cytokine production, cytotoxicity, and adhesion to various cell types. The protein is localized to the plasma membrane, and alternatively spliced transcript variants have been described, indicating diverse functional roles. CD58's involvement in immune responses highlights its significance in both normal physiological processes and disease states.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Ag3; CD305; CD58; CD58 antigen, (lymphocyte function-associated antigen 3); FLJ23181; FLJ43722; LAIR-1; LAIR1; Lymphocyte function-associated antigen 3; lymphocyte function-associated antigen 3 isoform delta D2; RP5-1086K13.2; Surface glycoprotein LFA-3; unnamed protein product
Gene Aliases: ag3; CD58; LFA-3; LFA3
UniProt ID: (Human) P19256
Entrez Gene ID: (Human) 965
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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