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Invitrogen
ICAM-1 Recombinant Rabbit Monoclonal Antibody (037)
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FIGURE: 1 / 2
ICAM-1 Antibody (MA5-29866) in ICC/IF
Immunofluorescence staining of rat ICAM-1 in rat splenocytes. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with ICAM-1 Recombinant Rabbit Monoclonal Antibody (37) (Product # MA5-29866, 1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).
Product Details
MA5-29866
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
037
Immunogen
Recombinant Rat ICAM-1/CD54 protein (Met1-Thr493)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Rat ICAM-1/CD54.
Target Information
ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD54; Human rhinovirus receptor; ICAM-1; Intercellular adhesion molecule 1; Ly 47; MALA2; sCD54; soluble CD54; Surface antigen of activated B cells
Gene Aliases: CD54; ICAM; Icam1
UniProt ID: (Rat) Q00238
Entrez Gene ID: (Rat) 25464
ovarian follicle development
response to hypoxia
response to amphetamine
T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell
acute inflammatory response to antigenic stimulus
T cell antigen processing and presentation
positive regulation of cellular extravasation
cell adhesion
leukocyte cell-cell adhesion
cell aging
sensory perception of sound
regulation of cell shape
response to ionizing radiation
response to sulfur dioxide
response to organic cyclic compound
single organismal cell-cell adhesion
membrane to membrane docking
regulation of cell adhesion
positive regulation of actin filament polymerization
cellular response to nutrient levels
response to lipopolysaccharide
cell adhesion mediated by integrin
response to gonadotropin
response to drug
response to amino acid
positive regulation of GTPase activity
adhesion of symbiont to host
positive regulation of nitric oxide biosynthetic process
response to ethanol
positive regulation of vasoconstriction
response to copper ion
receptor-mediated virion attachment to host cell
positive regulation of peptidyl-tyrosine phosphorylation
leukocyte migration
positive regulation of NF-kappaB transcription factor activity
negative regulation of calcium ion transport
establishment of endothelial barrier
positive regulation of ERK1 and ERK2 cascade
cellular response to lipopolysaccharide
cellular response to organic substance
cellular response to alkaloid
cellular response to glucose stimulus
cellular response to interleukin-1
cellular response to tumor necrosis factor
cellular response to hypoxia
establishment of Sertoli cell barrier
regulation of ruffle assembly
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
negative regulation of endothelial cell apoptotic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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