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Phospho-MKK7 (Ser271, Thr275) Recombinant Rabbit Monoclonal Antibody (MKK7S271T275-R4F9)
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FIGURE: 1 / 3
Phospho-MKK7 (Ser271, Thr275) Antibody (MA5-37005) in WB
Western blot analysis of Phospho-MKK7 (Ser271, Thr275) using THP1 cell extract. Samples were untreated or treated with sorbitol, and incubated in monoclonal Phospho-MKK7 (Ser271, Thr275) antibody (Product # MA5-37005) with a dilution of 0.1 µg/mL.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-37005
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
MKK7S271T275-R4F9
Immunogen
A synthetic phospho-peptide corresponding to residues surrounding Ser271/Thr275 of human phospho MKK7.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
10 µg
Purification
Protein A/G
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2, MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found, but only one transcript variant has been supported and defined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: a new family member of MAP kinase kinases; c-Jun N-terminal kinase kinase 2; JN; JNK kinase 2; JNK-activating kinase 2; JNKK 2; MAP kinase kinase; MAP kinase kinase 7; MAP2K4; MAPK/ERK kinase 4; MAPK/ERK kinase 7; MAPKK 4; MAPKK 7; MEK 4; MEK 7; MKK7alpha2; MKK7; MKK7beta1; MKK7; MKK7beta2; MKK7; MKK7gamma1; MKK7; MKK7gamma2; MKK7; protein kinase; protein kinase homolog; protein kinase, mitogen activated, kinase 7; SAPK kinase 1; SAPK kinase 4; SAPK/ERK kinase 1; SAPKK-1; SAPKK1; SEK1; stress-activated protein kinase kinase 4; unnamed protein product
Gene Aliases: 5930412N11Rik; JNKK; JNKK 2; JNKK2; MAPKK 7; MAPKK7; MEK; MEK 7; Mek7; MKK7; PRKMK7; SAPKK-4; SAPKK4; sek2
UniProt ID: (Human) O14733, (Mouse) Q8CE90, (Rat) Q4KSH7
Entrez Gene ID: (Human) 5609, (Mouse) 26400, (Rat) 363855
magnesium ion binding
protein kinase activity
MAP kinase kinase activity
protein tyrosine kinase activity
protein binding
ATP binding
JUN kinase kinase activity
enzyme binding
protein kinase binding
protein phosphatase binding
mitogen-activated protein kinase binding
protein serine kinase activity
MAP kinase activity
mitogen-activated protein kinase kinase kinase binding
molecular function activator activity
positive regulation of protein phosphorylation
protein phosphorylation
response to stress
response to osmotic stress
JNK cascade
response to heat
response to UV
response to wounding
positive regulation of telomere maintenance
response to tumor necrosis factor
regulation of neuron apoptotic process
positive regulation of neuron apoptotic process
positive regulation of transcription, DNA-templated
positive regulation of JNK cascade
stress-activated MAPK cascade
positive regulation of ERK1 and ERK2 cascade
cellular response to lipopolysaccharide
cellular response to interleukin-1
cellular response to osmotic stress
regulation of motor neuron apoptotic process
signal transduction
stress-activated protein kinase signaling cascade
Fc-epsilon receptor signaling pathway
positive regulation of MAPK cascade
cellular senescence
cellular response to sorbitol
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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