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          • Primary Antibodies ›
          • PTEN Antibodies

          Invitrogen

          Phospho-PTEN (Ser380) Recombinant Rabbit Monoclonal Antibody (PTENS380-NA9), FITC

          View all (23) PTEN antibodies

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          Cite Phospho-PTEN (Ser380) Recombinant Rabbit Monoclonal Antibody (PTENS380-NA9), FITC

          Additional Information:

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          • Antibody Testing Data (1)
          Phospho-PTEN (Ser380) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          Phospho-PTEN (Ser380) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          Phospho-PTEN (Ser380) Antibody (MA5-37052) in Flow

          Flow cytometry of Phospho-PTEN (Ser380) using A431 cells. Samples were incubated in monoclonal Phospho-PTEN (Ser380) antibody (Product # MA5-37052). Images correspond with the following conditions: untreated, unstained negative control (blue), untreated and stained (green), treated with lambda phosphatase and stained (red). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

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          Phospho-PTEN (Ser380) Antibody in Flow Cytometry (Flow)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Phospho-PTEN (Ser380) Recombinant Rabbit Monoclonal Antibody (PTENS380-NA9), FITC

          Product Details

          MA5-37052

          Applications
          Tested Dilution
          Publications

          Flow Cytometry (Flow)

          5 µL/1x10^6 cells
          -
          Product Specifications

          Species Reactivity

          Human, Mouse

          Host/Isotype

          Rabbit / IgG, kappa

          Expression System

          HEK293 cells

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          PTENS380-NA9

          Immunogen

          A synthetic phospho peptide corresponding to residues surrounding Ser380 of human phospho PTEN.
          3D Epitope / Immunogen

          Conjugate

          FITC FITC FITC
          • Unconjugated
          • APC 
          • PE 
          • Request custom conjugation

          Excitation/Emission Max

          498/517 nm View spectra spectra

          Form

          Liquid

          Purification

          Protein A/G

          Storage buffer

          PBS with 0.20% BSA

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2896987

          Target Information

          PTEN was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The PTEN gene is a phosphatidylinositol-3, 4, 5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. PTEN negatively regulates intracellular levels of phosphatidylinositol-3, 4, 5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. PTEN plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Also, PTEN may be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. The nuclear monoubiquitinated form possesses greater apoptotic potential, whereas the cytoplasmic nonubiquitinated form of PTEN induces less tumor suppressive ability.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

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          Cite this product

          Bioinformatics

          Protein Aliases: inositol polyphosphate 3-phosphatase; mitochondrial phosphatase and tensin protein alpha; mitochondrial PTENalpha; MMAC1 phosphatase and tensin homolog deleted on chromosome 10; mutated in multiple advanced cancers 1; N-terminal extended isoform of PTEN translated from an alternative ATT initiation codon in the 5'UTR; N-terminal extended PTEN isoform; uses an alternative ATT initiation codon in PTEN 5'UTR; phosphatidylinositol-3,4,5-trisphosphate3-phosphatase; phosphatase and tensin; phosphatase and tensin-like protein; phosphatidylinositol 3; phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN isoform 5; phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN isoform 6; phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN isoform 7; phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, isoform 21; phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, isoform 22; phosphatidylinositol-3; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; protein tyrosine phosphatase; protein tyrosine phosphatase and tensin homolog; PTEN; pten tumor suppressor; PTEN variant PTEN-K267Rfs*9; PTEN variant PTEN-L247*; PTEN variant PTEN-R234Afs*11; PTENepsilon; TEP1; transcript variant 1; unnamed protein product

          View more View less

          Gene Aliases: 10q23del; 2310035O07Rik; A130070J02Rik; B430203M17Rik; BZS; CWS1; DEC; GLM2; MHAM; MMAC1; PTEN1; PTENbeta; PTENgama; TEP1

          View more View less

          UniProt ID: (Human) P60484, (Mouse) O08586

          View more View less

          Entrez Gene ID: (Human) 5728, (Mouse) 19211

          View more View less

          Function(s)
          phosphatidylinositol-3-phosphatase activity phosphoprotein phosphatase activity protein serine/threonine phosphatase activity protein tyrosine phosphatase activity protein binding beta-catenin binding anaphase-promoting complex binding phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity phosphatase activity enzyme binding PDZ domain binding inositol-1,3,4,5,6-pentakisphosphate 3-phosphatase activity identical protein binding inositol-1,3,4,5-tetrakisphosphate 3-phosphatase activity phosphatidylinositol-3,4-bisphosphate 3-phosphatase activity phosphatidylinositol phosphate phosphatase activity molecular function inhibitor activity ubiquitin-specific protease binding ubiquitin ligase activator activity platelet-derived growth factor receptor binding protein kinase binding ionotropic glutamate receptor binding protein tyrosine kinase binding
          Process(es)
          protein dephosphorylation phosphatidylinositol biosynthetic process spindle assembly involved in female meiosis neuron-neuron synaptic transmission synapse assembly central nervous system development heart development learning or memory locomotory behavior positive regulation of cell proliferation negative regulation of cell proliferation regulation of signal transduction negative regulation of cell communication negative regulation of epithelial to mesenchymal transition regulation of neuron projection development negative regulation of neuron projection development cell migration dentate gyrus development central nervous system neuron axonogenesis negative regulation of signaling negative regulation of cell migration adult behavior regulation of protein stability central nervous system myelin maintenance negative regulation of peptidyl-serine phosphorylation multicellular organismal response to stress social behavior maternal behavior protein kinase B signaling locomotor rhythm regulation of cell differentiation negative regulation of osteoblast differentiation negative regulation of cell size positive regulation of transcription from RNA polymerase II promoter negative regulation of organ growth phosphatidylinositol dephosphorylation forebrain morphogenesis cell motility negative regulation of axonogenesis protein stabilization negative regulation of developmental process negative regulation of cellular component organization negative regulation of multicellular organismal process negative regulation of keratinocyte migration regulation of cell cycle negative regulation of focal adhesion assembly regulation of protein kinase B signaling negative regulation of protein kinase B signaling rhythmic synaptic transmission synapse maturation prepulse inhibition long term synaptic depression dendritic spine morphogenesis cellular response to electrical stimulus negative regulation of excitatory postsynaptic potential presynaptic membrane assembly postsynaptic density assembly positive regulation of intracellular signal transduction negative regulation of cell cycle G1/S phase transition negative regulation of wound healing, spreading of epidermal cells negative regulation of vascular smooth muscle cell proliferation positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of G1/S transition of mitotic cell cycle positive regulation of excitatory postsynaptic potential negative regulation of cellular senescence negative regulation of synaptic vesicle clustering angiogenesis osteoblast differentiation regulation of B cell apoptotic process apoptotic process memory gene expression positive regulation of gene expression positive regulation of cardiac muscle cell apoptotic process neuron projection development negative regulation of myelination regulation of synaptic transmission, GABAergic regulation of cellular component size positive regulation of interleukin-6 production positive regulation of tumor necrosis factor production cellular response to insulin stimulus regulation of myeloid cell apoptotic process cellular response to decreased oxygen levels positive regulation of apoptotic process negative regulation of apoptotic process endothelial cell migration cellular response to leptin stimulus negative regulation of protein kinase activity by regulation of protein phosphorylation positive regulation of neuron differentiation negative regulation of cell cycle platelet-derived growth factor receptor signaling pathway regulation of axon regeneration negative regulation of axon regeneration cardiac muscle tissue development brain morphogenesis negative regulation of epithelial cell proliferation negative regulation of phagocytosis negative regulation of cardiac muscle cell proliferation male mating behavior long-term synaptic potentiation regulation of cellular localization regulation of Fc receptor mediated stimulatory signaling pathway prostate gland growth negative regulation of androgen receptor signaling pathway negative regulation of dendritic spine morphogenesis positive regulation of ERK1 and ERK2 cascade cellular response to ethanol cellular response to hypoxia inositol phosphate catabolic process negative regulation of ribosome biogenesis negative regulation of defense response to bacterium negative regulation of potassium ion transmembrane transporter activity negative regulation of dendrite extension negative regulation of TORC1 signaling cellular response to insulin-like growth factor stimulus negative regulation of receptor activity positive regulation of apoptotic signaling pathway
          It has to be done as per old AB suggested Products section.

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