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Invitrogen

Phospho-PKC alpha (Thr497) Recombinant Rabbit Monoclonal Antibody (PKCaT497-F1)

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Phospho-PKC alpha (Thr497) Recombinant Rabbit Monoclonal Antibody (PKCaT497-F1)

Product Details

MA5-37116

Applications
Tested Dilution
Publications

Western Blot (WB)

Assay-dependent
-

Flow Cytometry (Flow)

1-1,000 ng/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG, kappa

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

PKCaT497-F1

Immunogen

A synthetic phospho-peptide corresponding to residues surrounding Thr497 of human phospho PKC alpha.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Amount

100 µg

Purification

Protein A/G

Storage buffer

PBS with 0.10% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2897050

Target Information

The PKC family of serine/threonine kinases, including PRKCA (PKC alpha), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent. Protein kinase C (PKC) can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: AAG6; aging-associated gene 6; EC 2.7.11.13; FHC; FTH; FTHL6; kinase PKC-alpha; KPCA; MGC104426; PIG15; PKC III; PKC-A; PKC-alpha; PKC-III; PLIF; protein kinase C alpha-polypeptide; protein kinase c-alpha; unnamed protein product

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Gene Aliases: AAG6; PKC-alpha; PKCA; PKCalpha; PKCI+/-

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UniProt ID: (Human) P17252, (Mouse) P20444, (Rat) P05696

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Entrez Gene ID: (Human) 5578, (Mouse) 18750, (Rat) 24680

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Function(s)
protein kinase activity protein serine/threonine kinase activity protein kinase C activity calcium-dependent protein kinase C activity receptor binding integrin binding protein binding ATP binding zinc ion binding enzyme binding diacylglycerol binding histone kinase activity (H3-T6 specific) protein serine kinase activity lipid binding
Process(es)
response to reactive oxygen species negative regulation of protein phosphorylation positive regulation of protein phosphorylation positive regulation of endothelial cell proliferation regulation of the force of heart contraction chondrocyte differentiation desmosome assembly chromatin remodeling protein phosphorylation negative regulation of protein kinase activity cellular calcium ion homeostasis regulation of muscle contraction learning or memory negative regulation of cell proliferation positive regulation of signal transduction positive regulation of endothelial cell migration positive regulation of cardiac muscle hypertrophy negative regulation of glucose transport negative regulation of translation central nervous system neuron axonogenesis signal transduction involved in regulation of gene expression positive regulation of cell migration neutrophil chemotaxis positive regulation of lipopolysaccharide-mediated signaling pathway response to estradiol negative regulation of glial cell apoptotic process intracellular signal transduction peptidyl-serine autophosphorylation proteasome-mediated ubiquitin-dependent protein catabolic process negative regulation of MAPK cascade protein kinase B signaling positive regulation of blood vessel endothelial cell migration positive regulation of cellular component biogenesis response to ethanol positive regulation of macrophage differentiation positive regulation of angiogenesis positive regulation of bone resorption positive regulation of cell adhesion positive regulation of exocytosis positive regulation of mitotic cell cycle negative regulation of glucose import negative regulation of insulin receptor signaling pathway protein autophosphorylation positive regulation of smooth muscle cell proliferation positive regulation of inflammatory response regulation of peptidyl-tyrosine phosphorylation induction of positive chemotaxis positive regulation of developmental process positive regulation of multicellular organismal process positive regulation of protein kinase B signaling positive regulation of synapse assembly renal sodium ion absorption positive regulation of ERK1 and ERK2 cascade protein kinase C signaling response to interleukin-1 cellular response to carbohydrate stimulus regulation of platelet aggregation intrinsic apoptotic signaling pathway positive regulation of angiotensin-activated signaling pathway negative regulation of cytokine production involved in inflammatory response response to phorbol 13-acetate 12-myristate regulation of synaptic vesicle exocytosis positive regulation of dense core granule biogenesis mitotic nuclear envelope disassembly response to mechanical stimulus response to toxic substance positive regulation of insulin secretion response to peptide hormone regulation of mRNA stability regulation of transport response to corticosterone negative regulation of dopamine receptor signaling pathway apoptotic signaling pathway positive regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway regulation of exocytosis establishment of protein localization
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