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Phospho-PKC alpha (Thr497) Recombinant Rabbit Monoclonal Antibody (PKCaT497-F1), FITC

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Phospho-PKC alpha (Thr497) Recombinant Rabbit Monoclonal Antibody (PKCaT497-F1), FITC

Product Details

MA5-37118

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

5 µL/1x10^6 cells
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG, kappa

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

PKCaT497-F1

Immunogen

A synthetic phospho-peptide corresponding to residues surrounding Thr497 of human phospho PKC alpha.
3D Epitope / Immunogen

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra spectra

Form

Liquid

Purification

Protein A/G

Storage buffer

PBS with 0.20% BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2897052

Target Information

The PKC family of serine/threonine kinases, including PRKCA (PKC alpha), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent. Protein kinase C (PKC) can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: AAG6; aging-associated gene 6; EC 2.7.11.13; FHC; FTH; FTHL6; kinase PKC-alpha; KPCA; MGC104426; PIG15; PKC III; PKC-A; PKC-alpha; PKC-III; PLIF; Protein kinase C alpha type; protein kinase C alpha-polypeptide; protein kinase c-alpha; unnamed protein product

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Gene Aliases: AAG6; AI875142; PKC-alpha; PKCA; PKCalpha; PKCI+/-; PRKACA; PRKCA

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UniProt ID: (Human) P17252, (Mouse) P20444, (Rat) P05696

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Entrez Gene ID: (Human) 5578, (Mouse) 18750, (Rat) 24680

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Function(s)
nucleotide binding protein kinase activity protein serine/threonine kinase activity protein kinase C activity calcium-dependent protein kinase C activity receptor binding integrin binding protein binding ATP binding zinc ion binding kinase activity transferase activity enzyme binding diacylglycerol binding histone kinase activity (H3-T6 specific) metal ion binding protein serine kinase activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase
Process(es)
response to reactive oxygen species angiogenesis positive regulation of endothelial cell proliferation desmosome assembly chromatin remodeling protein phosphorylation apoptotic process mitotic nuclear envelope disassembly cell adhesion learning or memory response to mechanical stimulus response to toxic substance positive regulation of signal transduction positive regulation of endothelial cell migration positive regulation of cardiac muscle hypertrophy negative regulation of translation central nervous system neuron axonogenesis signal transduction involved in regulation of gene expression positive regulation of cell migration positive regulation of lipopolysaccharide-mediated signaling pathway positive regulation of insulin secretion response to estradiol negative regulation of glial cell apoptotic process intracellular signal transduction proteasome-mediated ubiquitin-dependent protein catabolic process response to peptide hormone regulation of mRNA stability protein kinase B signaling positive regulation of blood vessel endothelial cell migration response to ethanol positive regulation of macrophage differentiation positive regulation of angiogenesis positive regulation of bone resorption positive regulation of cell adhesion positive regulation of exocytosis positive regulation of mitotic cell cycle positive regulation of smooth muscle cell proliferation regulation of transport response to corticosterone positive regulation of protein kinase B signaling positive regulation of synapse assembly renal sodium ion absorption positive regulation of ERK1 and ERK2 cascade protein kinase C signaling response to interleukin-1 regulation of platelet aggregation apoptotic signaling pathway positive regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway positive regulation of angiotensin-activated signaling pathway negative regulation of cytokine production involved in inflammatory response positive regulation of cytokine production involved in inflammatory response response to phorbol 13-acetate 12-myristate positive regulation of dense core granule biogenesis inactivation of MAPK activity negative regulation of protein phosphorylation positive regulation of protein phosphorylation regulation of the force of heart contraction chondrocyte differentiation negative regulation of protein kinase activity cellular calcium ion homeostasis regulation of muscle contraction negative regulation of cell proliferation phosphorylation peptidyl-serine phosphorylation peptidyl-threonine phosphorylation neutrophil chemotaxis histone H3-T6 phosphorylation peptidyl-serine autophosphorylation negative regulation of heart contraction negative regulation of glucose import negative regulation of insulin receptor signaling pathway protein autophosphorylation regulation of receptor-mediated endocytosis positive regulation of inflammatory response regulation of peptidyl-tyrosine phosphorylation induction of positive chemotaxis cellular response to carbohydrate stimulus intrinsic apoptotic signaling pathway aging response to organic cyclic compound establishment of protein localization response to antibiotic
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