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CD45 Monoclonal Antibody (2D1), PerCP

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CD45 Monoclonal Antibody (2D1), PerCP

Product Details

MA5-38733

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

10 µL/10^6 cells
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

2D1

Immunogen

Human peripheral blood mononuclear cells
3D Epitope / Immunogen

Conjugate

PerCP PerCP PerCP

Excitation/Emission Max

482/675 nm View spectra spectra

Form

Liquid

Concentration

0.09 mg/mL

Purification

Size-exclusion chromatography

Storage buffer

PBS with 0.2% BSA

Contains

15mM sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2898645

Product Specific Information

This antibody reacts with an extracellular epitope of all alternative forms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except from erythrocytes and platelets.

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD45 antigen; glycoprotein 180; protein tyrosine phosphatase; protein tyrosine phosphatase, receptor type, c polypeptide; T200 glycoprotein; T200 leukocyte common antigen; T220 and B220; unnamed protein product

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Gene Aliases: B220; CD45; CD45R; GP180; IMD105; L-CA; LCA; LY5; T200

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UniProt ID: (Human) P08575

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Entrez Gene ID: (Human) 5788

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Function(s)
protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity receptor binding protein binding heparin binding protein kinase binding protein tyrosine kinase inhibitor activity ankyrin binding spectrin binding heparan sulfate proteoglycan binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter natural killer cell differentiation negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation positive regulation of immunoglobulin production negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration signal transduction cell surface receptor signaling pathway response to gamma radiation regulation of gene expression dephosphorylation B cell differentiation T cell differentiation positive regulation of B cell proliferation regulation of interleukin-8 production negative regulation of interleukin-2 production positive regulation of interleukin-2 production positive regulation of tumor necrosis factor production B cell proliferation positive regulation of T cell proliferation T cell activation positive regulation of MAPK cascade cell cycle phase transition plasma membrane raft distribution positive regulation of protein kinase activity negative regulation of JAK-STAT cascade bone marrow development stem cell development regulation of phagocytosis positive regulation of phagocytosis positive regulation of calcium-mediated signaling T cell receptor signaling pathway B cell receptor signaling pathway regulation of T cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of Fc receptor mediated stimulatory signaling pathway negative regulation of ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade negative regulation of interleukin-4-mediated signaling pathway negative regulation of microglial cell activation response to aldosterone DN2 thymocyte differentiation positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation
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