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Invitrogen
Alpha A Crystallin Monoclonal Antibody (1H3.B8), PerCP
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FIGURE: 1 / 2
Alpha A Crystallin Antibody (MA5-45174) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with Alpha A Crystallin monoclonal antibody (Product # MA5-45174) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1... View More
Product Details
MA5-45174
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
1H3.B8
Immunogen
Native Alpha Crystallin
Conjugate
Excitation/Emission Max
482/675 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
200 µg
Purification
Protein G
Storage buffer
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
0.5 µg/mL was sufficient for detection of 100 ng purified alphaA crystalline by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.|Detects approximately 20kDa. Does not cross-react with alpha B-crystallin, beta L-crystallin, beta H- crystallin, gamma -crystallin, HSP25, HSP27 or HSP47 proteins.
Target Information
Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products-alpha-A and alpha-B. There are at least 5 different proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, but there are at least 5 gamma crystallins identified in bovine and rat lens. Alpha-Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha-B crystallin has been linked to Alexander's disease where it accumulates in brain cells of those afflicted.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Acry 1; alpha-A-crystallin; Alpha-crystallin A chain; CRYA 1; CRYAA; Crystallin Alpha 1; crystallin, alpha 1; Crystallin, alpha polypeptide A; crystallin, alpha-1; Heat shock protein beta-4; Heat shock protein family B member 4; HSPB 4; HspB4; human alphaA-crystallin (CRYA1); lens opacity 18
Gene Aliases: Acry-1; Crya-1; CRYA1; CRYAA; CRYAA2; CTRCT9; DAcry-1; HSPB4; lop18
UniProt ID: (Bovine) P02470, (Human) P02489, (Rat) P24623, (Mouse) P24622
Entrez Gene ID: (Bovine) 281718, (Human) 1409, (Rat) 24273, (Mouse) 12954, (Human) 102724652
response to hypoxia
positive regulation of protein phosphorylation
mitochondrion organization
actin filament organization
microtubule-based process
tubulin complex assembly
negative regulation of gene expression
positive regulation of cell growth
protein refolding
response to hydrogen peroxide
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
embryonic camera-type eye morphogenesis
apoptotic process involved in morphogenesis
response to UV-A
lens fiber cell morphogenesis
lens development in camera-type eye
lens morphogenesis in camera-type eye
protein folding
apoptotic process
visual perception
response to lead ion
negative regulation of intracellular transport
response to drug
camera-type eye development
negative regulation of apoptotic process
protein homooligomerization
response to glucocorticoid
response to heat
protein stabilization
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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