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Invitrogen
Alpha A Crystallin Monoclonal Antibody (1H3.B8)
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FIGURE: 1 / 2
Alpha A Crystallin Antibody (MA5-27582) in ICC/IF
Immunofluorescent analysis of Alpha A Crystallin in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with Alpha A Crystallin monoclonal antibody (Product # MA5-27582) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5... View More
Product Details
MA5-27582
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
1H3.B8
Immunogen
Native Human Alpha Crystallin
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
0.5 µg/mL was sufficient for detection of 100 ng purified alphaA crystalline by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.|Detects approximately 20kDa. Does not cross-react with alpha B-crystallin, beta L-crystallin, beta H- crystallin, gamma -crystallin, HSP25, HSP27 or HSP47 proteins.
Target Information
Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products-alpha-A and alpha-B. There are at least 5 different proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, but there are at least 5 gamma crystallins identified in bovine and rat lens. Alpha-Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha-B crystallin has been linked to Alexander's disease where it accumulates in brain cells of those afflicted.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Acry 1; alpha-A-crystallin; Alpha-crystallin A chain; CRYA 1; CRYAA; Crystallin Alpha 1; crystallin, alpha 1; Crystallin, alpha polypeptide A; crystallin, alpha-1; Heat shock protein beta-4; Heat shock protein family B member 4; HSPB 4; HspB4; human alphaA-crystallin (CRYA1); lens opacity 18
Gene Aliases: Acry-1; Crya-1; CRYA1; CRYAA; CRYAA2; CTRCT9; DAcry-1; HSPB4; lop18
UniProt ID: (Bovine) P02470, (Human) P02489, (Rat) P24623, (Mouse) P24622
Entrez Gene ID: (Bovine) 281718, (Human) 1409, (Rat) 24273, (Mouse) 12954, (Human) 102724652
response to hypoxia
positive regulation of protein phosphorylation
mitochondrion organization
actin filament organization
microtubule-based process
tubulin complex assembly
negative regulation of gene expression
positive regulation of cell growth
protein refolding
response to hydrogen peroxide
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
embryonic camera-type eye morphogenesis
apoptotic process involved in morphogenesis
response to UV-A
lens fiber cell morphogenesis
lens development in camera-type eye
lens morphogenesis in camera-type eye
protein folding
apoptotic process
visual perception
response to lead ion
negative regulation of intracellular transport
response to drug
camera-type eye development
negative regulation of apoptotic process
protein homooligomerization
response to glucocorticoid
response to heat
protein stabilization
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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