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Invitrogen

HSP70 Monoclonal Antibody (3A3), APC

View all (64) HSP70 antibodies

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Product Details

MA5-45214

Applications
Tested Dilution
Publications

Western Blot (WB)

1:5,000
-

Immunocytochemistry (ICC/IF)

1:500
-
Product Specifications

Species Reactivity

Amphibian, Arthropod, Bacteria, Chicken, Fruit fly, Fish, Human, Mouse, Prawn, Rat, Yeast

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3A3

Immunogen

Human recombinant HSP70 overexpressed in E.coli

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Protein G

Storage buffer

95.46mM phosphate/2.48mM MES, pH 7.2, with 2mM EDTA

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2931668

Product Specific Information

0.2 µg/mL of MA5-45214 was sufficient for detection of HSP70 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 70kDa. May detect HSP70, HSC70, p75 and HSP72.

Target Information

HSP70 is a Heat shock protein (HSP) which are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs that include HSP70, there are HSP90 and HSP100. The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75 kDa protein that is found within the mitochondria. Further, HSP70 is encoded by an intronless gene and, in conjunction with other heat shock proteins, HSP70 stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and organelles. HSP70 is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The HSP70 gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 68 kDa heat shock protein; 70 kilodalton heat shock protein; 87A locus; 87C locus; ARABIDOPSIS HEAT SHOCK PROTEIN 70; ATHSP70; CG31359-PA; CG31366-PA; dnaK-type molecular chaperone HSP70-1; epididymis secretory protein Li 103; epididymis secretory sperm binding protein; epididymis secretory sperm binding protein Li 5a; heat shock 10kDa protein 1-like; heat shock 70; Heat shock 70 kDa protein 1; heat shock 70 kDa protein 1-Hom; heat shock 70 kDa protein 1/2; Heat shock 70 kDa protein 1A; heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1B; heat shock 70 kDa protein 1L; Heat shock 70 kDa protein 2; heat shock 70 kDa protein 3; heat shock 70 kDa-like protein 1; heat shock 70-related protein APG-2; heat shock 70kD protein 1A; heat shock 70kD protein 1B; heat shock 70kD protein 2; heat shock 70kD protein 4; heat shock 70kD protein-like 1; heat shock 70kDa protein 1-like; heat shock 70kDa protein 1A; heat shock 70kDa protein 1B; heat shock 70kDa protein 2; heat shock 70kDa protein 4; Heat shock protein; heat shock protein 1-like; heat shock protein 1A; heat shock protein 1B; heat shock protein 2; heat shock protein 4; heat shock protein 70; heat shock protein 70-1; Heat shock protein 70-1l; heat shock protein 70.1; heat shock protein 70.2; heat shock protein alpha 2; heat shock protein cognate 70, testis; heat shock protein family A member 1A; heat shock protein family A member 1B; heat shock protein family A member 1L; heat shock protein family A member 2; heat shock protein family A member 4; heat shock protein family H member 2; heat shock protein, 110 kDa; heat shock protein, 70 kDa 1; heat shock protein, 70 kDa 2; heat shock protein, 70 kDa 3; heat shock protein-70; heat shock-induced protein; heat shock-related protein; heat shock-related protein hsc70t; heat-shock protein 70; HEL S 103; HSP; HSP70 1; HSP70 1B; HSP70 2; hsp70 87A; HSP70 AceyltK77; hsp70 RY; HSP70-1/HSP70-2; HSP70.1/2; HSP70.1/HSP70.2; HSP70.3; Hsp70Aa-PA; Hsp70Bb-PA; HSPA2; HSPA4; Hspa70; HST; inducible heat shock protein 70; IRP94; ischemia responsive 94 kDa protein; LP05203p; LP05233p; LP08776p; protein 70; spermatid-specific heat shock protein 70; testis-specific heat shock protein-related gene hst70; unnamed protein product

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Gene Aliases: 70kDa; 87A; APG-2; Bb; CG31359; CG31366; dHSP70; dhsp70Aa; Dm-hsp70; Dmel\CG31359; Dmel\CG31366; Dmel_CG31359; Dmel_CG31366; DmHSP70AA; DMHSP7D1; HEL-S-103; HEL-S-5a; HS24/P52; hs70; Hsc70t; HSP 70; Hsp-70; Hsp-70Aa; Hsp110; hsp68; HSP70; hsp70 87A; hsp70 87C; hsp70 Aa; hsp70(87A); Hsp70(87C); HSP70-1; HSP70-1A; HSP70-1B; HSP70-1L; HSP70-2; HSP70-3; HSP70-HOM; HSP70.1; HSP70.2; Hsp70.3; Hsp70A; hsp70A1; HSP70A2; Hsp70A7d; hsp70Aa; Hsp70B; HSP70B1; Hsp70bb; Hsp70Bbc; Hsp70Bc; HSP70I; Hsp70RY; HSP70T; HSP72; HSPA1; HSPH2; Hspt70; Hst70; HSX70; hum70t; irp94; mKIAA4025; Msh5; RY

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UniProt ID: (Human) P0DMV8, (Human) P34931, (Human) P54652, (Human) P34932, (Fruit fly) P02825, (Fruit fly) Q9BIR7, (Rat) P0DMW0, (Mouse) Q61696, (Mouse) P17879, (Mouse) P16627, (Rat) P55063, (Rat) P14659, (Mouse) P17156, (Rat) O88600

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Entrez Gene ID: (Human) 3303, (Human) 3304, (Human) 3305, (Human) 3306, (Chicken) 416339, (Human) 3308, (Fruit fly) 48581, (Fruit fly) 48582, (Rat) 24472, (Mouse) 193740, (Mouse) 15511, (Mouse) 15482, (Rat) 24963, (Rat) 60460, (Mouse) 15512, (Rat) 266759, (Mouse) 15525

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Function(s)
G-protein coupled receptor binding transcription corepressor activity RNA binding receptor binding protein binding ATP binding ATPase activity enzyme binding heat shock protein binding ubiquitin protein ligase binding histone deacetylase binding protein binding involved in protein folding cadherin binding receptor agonist activity misfolded protein binding C3HC4-type RING finger domain binding disordered domain specific binding transcription regulator inhibitor activity ATP-dependent protein disaggregase activity tau protein binding chaperone binding glycolipid binding adenyl-nucleotide exchange factor activity unfolded protein binding protease binding NF-kappaB binding death receptor agonist activity macromolecular complex binding RNA polymerase II sequence-specific DNA binding transcription factor binding
Process(es)
kidney development negative regulation of protein phosphorylation protein folding negative regulation of gene expression protein import into mitochondrial matrix negative regulation of apoptotic process positive regulation of angiogenesis chaperone-mediated protein complex assembly neuron apoptotic process regulation of microglial cell activation negative regulation of transcription from RNA polymerase II promoter mRNA catabolic process response to unfolded protein lysosomal transport negative regulation of cell proliferation positive regulation of gene expression negative regulation of cell growth negative regulation of transforming growth factor beta receptor signaling pathway endoplasmic reticulum unfolded protein response regulation of protein ubiquitination negative regulation of protein ubiquitination positive regulation of proteasomal ubiquitin-dependent protein catabolic process positive regulation of interleukin-8 production positive regulation of RNA splicing cellular response to oxidative stress cellular response to heat cellular response to unfolded protein protein refolding positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of erythrocyte differentiation ATP metabolic process protein stabilization cellular heat acclimation positive regulation of nucleotide-binding oligomerization domain containing 2 signaling pathway cellular response to steroid hormone stimulus positive regulation of microtubule nucleation negative regulation of inclusion body assembly negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway regulation of mitotic spindle assembly negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway positive regulation of tumor necrosis factor-mediated signaling pathway negative regulation of extrinsic apoptotic signaling pathway in absence of ligand male meiosis spermatogenesis spermatid development response to heat response to cold response to hypoxia vesicle-mediated transport heat shock-mediated polytene chromosome puffing chaperone mediated protein folding requiring cofactor telomere maintenance positive regulation of T cell mediated cytotoxicity positive regulation of T cell mediated cytotoxicity directed against tumor cell target response to ischemia DNA repair defense response binding of sperm to zona pellucida response to mechanical stimulus neuron differentiation negative regulation of myeloid cell apoptotic process response to ethanol positive regulation of neuron differentiation negative regulation of vasoconstriction negative regulation of release of cytochrome c from mitochondria positive regulation of establishment of protein localization to mitochondrion biological_process response to stress positive regulation of protein phosphorylation male meiosis I positive regulation of G2/M transition of mitotic cell cycle synaptonemal complex disassembly
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