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Invitrogen

HSP70 Monoclonal Antibody (3A3), PerCP

View all (64) HSP70 antibodies

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Product Details

MA5-45216

Applications
Tested Dilution
Publications

Western Blot (WB)

1:5,000
-

Immunohistochemistry (IHC)

Assay-dependent
-

Immunocytochemistry (ICC/IF)

1:500
-

Immunoprecipitation (IP)

2 µg
-

Antibody Microarray (Ab Microarray)

Assay-dependent
-
Product Specifications

Species Reactivity

Amphibian, Arthropod, Bacteria, Chicken, Fruit fly, Fish, Human, Mouse, Prawn, Rat, Yeast

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3A3

Immunogen

Human recombinant HSP70 overexpressed in E.coli

Conjugate

PerCP PerCP PerCP

Excitation/Emission Max

482/675 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Protein G

Storage buffer

95.46mM phosphate/2.48mM MES, pH 7.2, with 2mM EDTA

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2931670

Product Specific Information

0.2 µg/mL of MA5-45216 was sufficient for detection of HSP70 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 70kDa. May detect HSP70, HSC70, p75 and HSP72.

Target Information

HSP70 is a Heat shock protein (HSP) which are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs that include HSP70, there are HSP90 and HSP100. The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75 kDa protein that is found within the mitochondria. Further, HSP70 is encoded by an intronless gene and, in conjunction with other heat shock proteins, HSP70 stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and organelles. HSP70 is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The HSP70 gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 68 kDa heat shock protein; 70 kilodalton heat shock protein; 87A locus; 87C locus; ARABIDOPSIS HEAT SHOCK PROTEIN 70; ATHSP70; CG31359-PA; CG31366-PA; heat shock 70; Heat shock 70 kDa protein 1; Heat shock 70 kDa protein 1-Hom; Heat shock 70 kDa protein 1-like; heat shock 70 kDa protein 1/2; Heat shock 70 kDa protein 1A; heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1B; Heat shock 70 kDa protein 1L; Heat shock 70 kDa protein 2; Heat shock 70 kDa protein 3; Heat shock 70 kDa protein 4; Heat shock 70 kDa protein Aa; Heat shock 70 kDa protein Ab; Heat shock 70 kDa-like protein 1; Heat shock 70-related protein APG-2; heat shock 70kD protein 1B; heat shock 70kDa protein 1-like; heat shock 70kDa protein 1A; heat shock 70kDa protein 1B; heat shock 70kDa protein 2; heat shock 70kDa protein 4; Heat shock protein; heat shock protein 70; Heat shock protein 70 Aa; Heat shock protein 70 Ab; heat shock protein 70-1; Heat shock protein 70-1l; heat shock protein 70.1; Heat shock protein 70.2; Heat shock protein 70Bb; Heat shock protein 70Bc; heat shock protein alpha 2; heat shock protein cognate 70, testis; Heat shock protein family A member 1A; Heat shock protein family A member 1B; Heat shock protein family A member 1L; Heat shock protein family A member 2; Heat shock protein family H member 2; heat shock protein, 110 kDa; heat shock protein, 70 kDa 1; heat shock protein, 70 kDa 2; heat shock protein, 70 kDa 3; heat shock protein-70; Heat shock-related 70 kDa protein 2; heat shock-related protein hsc70t; heat-shock protein 70; HEL S 103; HSP; Hsp68; HSP70 1; HSP70 1B; HSP70 2; hsp70 87A; HSP70 AceyltK77; HSP70-1; HSP70-1/HSP70-2; HSP70-2; HSP70-87A7; HSP70-87C1; HSP70-Hom; HSP70.1; HSP70.1/2; HSP70.1/HSP70.2; HSP70.3; Hsp70Aa-PA; Hsp70Bb-PA; HSP70RY; HSPA2; HSPA4; Hspa70; HST; inducible heat shock protein 70; Ischemia responsive 94 kDa protein; LP05203p; LP05233p; LP08776p; Major heat shock 70 kDa protein Bb; Major heat shock 70 kDa protein Bc; protein 70; Spermatid-specific heat shock protein 70; Testis-specific heat shock protein-related; testis-specific heat shock protein-related gene hst70

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Gene Aliases: 70kDa; 87A; AI317151; APG-2; APG2; Bb; CG18743; CG31359; CG31366; CG6489; dHSP70; dhsp70Aa; Dm-hsp70; Dmel\CG31359; Dmel\CG31366; Dmel_CG31359; Dmel_CG31366; DmHSP70AA; DMHSP7D1; Hcp70.1; Hcp70.2; hs70; Hsc70t; HSP 70; Hsp-70; Hsp-70Aa; Hsp110; hsp68; HSP70; hsp70 87A; hsp70 87C; hsp70 Aa; hsp70(87A); Hsp70(87C); Hsp70-1; Hsp70-2; Hsp70-3; Hsp70.1; HSP70.2; Hsp70.3; Hsp70A; Hsp70A1; HSP70A2; Hsp70A7d; Hsp70Aa; Hsp70Ab; Hsp70B; Hsp70Bb; Hsp70Bbc; Hsp70Bc; Hsp70RY; HSP72; HSPA1; HSPA1A; HSPA1B; HSPA1L; HSPA2; HSPA4; HSPH2; Hspt70; Hst70; HSX70; Irp94; mKIAA4025; Msh5

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UniProt ID: (Fruit fly) P02825, (Fruit fly) Q9BIR7, (Mouse) Q61696, (Rat) P0DMW0, (Mouse) P17879, (Rat) P55063, (Mouse) P16627, (Rat) P14659, (Mouse) P17156, (Rat) O88600

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Entrez Gene ID: (Chicken) 416339, (Fruit fly) 48581, (Fruit fly) 48582, (Mouse) 193740, (Rat) 24472, (Rat) 294254, (Mouse) 15511, (Rat) 24963, (Mouse) 15482, (Rat) 60460, (Mouse) 15512, (Mouse) 15525, (Rat) 266759

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Function(s)
ATP binding ATPase activity heat shock protein binding protein binding involved in protein folding unfolded protein binding misfolded protein binding G-protein coupled receptor binding protease binding double-stranded RNA binding enzyme binding ubiquitin protein ligase binding ATPase activity, coupled poly(A) RNA binding protein N-terminus binding NF-kappaB binding C3HC4-type RING finger domain binding protein binding molecular_function nucleotide binding glycolipid binding RNA polymerase II transcription factor binding protein complex binding activating transcription factor binding
Process(es)
binding of sperm to zona pellucida biological_process protein refolding telomere maintenance DNA repair protein folding response to heat response to hypoxia response to unfolded protein vesicle-mediated transport cellular response to unfolded protein heat shock-mediated polytene chromosome puffing chaperone mediated protein folding requiring cofactor negative regulation of protein phosphorylation positive regulation of gene expression negative regulation of gene expression positive regulation of protein binding negative regulation of apoptotic process negative regulation of DNA binding protein import into mitochondrial outer membrane chaperone-mediated protein complex assembly negative regulation of cell death response to ischemia mRNA catabolic process negative regulation of cell proliferation response to radiation response to mechanical stimulus regulation of cell death neuron differentiation negative regulation of cell growth negative regulation of protein ubiquitination cellular response to heat response to ethanol positive regulation of erythrocyte differentiation positive regulation of neuron differentiation cellular heat acclimation negative regulation of inclusion body assembly negative regulation of extrinsic apoptotic signaling pathway in absence of ligand kidney development positive regulation of angiogenesis positive regulation of protein phosphorylation male meiosis male meiosis I multicellular organism development spermatid development positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G2/M transition of mitotic cell cycle synaptonemal complex disassembly positive regulation of T cell mediated cytotoxicity defense response negative regulation of myeloid cell apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process negative regulation of vasoconstriction spermatogenesis cell differentiation positive regulation of protein targeting to mitochondrion
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