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Invitrogen
TXNDC5 Monoclonal Antibody (2E7/7), APC
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FIGURE: 1 / 1
TXNDC5 Antibody (MA5-45305) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human cervical cancer cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with EndoPDI monoclonal antibody (Product # MA5-45305) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was DAPI (blue) nuclear stain at 1:5,000 for 5 min RT. Localization: Nucleus, Endoplasmic Reticulum, Endoplasmic Reticulum Lumen. Magnification: 60X.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-45305
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
2E7/7
Immunogen
Synthesized peptide - 12 amino acids long (ADGEDGQDPHSK) corresponding to residues 52 - 63 of the EndoPDI protein
Conjugate
APC
APC
APC
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-45305 was sufficient for detection of EndoPDI in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti -mouse IgG:HRP as the secondary antibody.|Detects approximately 48kDa.
Target Information
This gene encodes a protein-disulfide isomerase. Its expression is induced by hypoxia and its role may be to protect hypoxic cells from apoptosis. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: endoplasmic reticulum protein ERp46; endoplasmic reticulum resident protein 46; endothelial protein disulphide isomerase; epididymis secretory sperm binding protein; ER protein 46; FLJ21353; FLJ90810; MGC3178; protein disulfide isomerase family A, member 15; thiore; thioredoxin domain containing 5 (endoplasmic reticulum); thioredoxin related protein; thioredoxin-like protein p46; unnamed protein product
Gene Aliases: ENDOPDI; ERP46; HCC-2; HCC2; PDIA15; STRF8; UNQ364
UniProt ID: (Human) Q8NBS9
Entrez Gene ID: (Human) 81567
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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